VSIG2 (V-set and Ig domain-containing protein 2; also CTM and CT-like protein) is presumably a 50-60kD member of the CTX family of proteins. It shows expression in stomach and prostate by Northern blot, and likely participates in cell adhesion. Human VSIG2 precursor is 327aa in length. It is a type I transmembrane (glyco)protein that contains a 220aa extracellular domain (ECD) (aa24-243) and a 63aa cytoplasmic region. The ECD contains one V-type (aa24-137) and one C2-type Ig-like domain (aa144-233). One potential splice variant exists that shows a deletion of aa285-327. over aa 24-243, human VSIG2 is 85% aa identical to mouse VSIG2.
Applications:
Suitable for use in ELISA and Western Blot. Other applications not tested.
Recommended Dilution:
ELISA: 0.5-1ug/ml
Western Blot: 2ug/ml
Optimal dilutions to be determined by the researcher.
Hybridoma:
Mouse myeloma cells with B cells obtained from a mouse immunized with purified, NS0-derived, recombinant human VSIG2 (aa1-243).
Storage and Stability:
Lyophilized powder may be stored at -20 degrees C for short-term only. Reconstitute with sterile 40-50% glycerol, aliquot and store at -20 degrees C. Reconstituted product is stable for 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
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