WDR5, Recombinant, Human, His-Tag (WD Repeat Domain 5, BIG3, BIG-3, BMP2-induced 3-kb Gene Protein, SWD3, WD Repeat-containing Protein 5)

WD-repeat protein 5 (WDR5, also designated BMP-2-induced gene 3 kb or BIG-3) belongs to the family of WD-40 repeat proteins, and is essential for vertebrate development, Hox gene activation and global H3K4 trimethylation. WDR5 is a conserved subunit of Trithorax (TRX) histone methyltransferase complexes that selectively binds to dimethylated Lys4 (K4me2) in Histone H3 to promote K4 trimethylation by TRX. It is expressed in osteoblasts, chondrocytes, osteocytes and marrow stromal cells. The WDR5 protein contains 7 WD-repeats, which may play a role in its function of accelerating osteoblast differentiation.\n\nSource:\nRecombinant corresponding to aa2-334 from human WDR5, fused to His-tag at N-terminal, expressed in Sf9 insect cells via a baculovirus expression system.\n\nMolecular Weight:\n~37.5kD\n\nApplications: \nSuitable for use in the study of enzyme kinetics, screening inhibitors, and selectivity profiling. Other applications not tested.\n\nRecommended Dilution:\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nAliquot to avoid repeated freezing and thawing and store at -70 degrees C. Aliquots are stable for 6 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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SPECIFICATIONS

Catalog Number

137400

Size

100ug

Form

Supplied as a liquid in 40mM Tris-HCl, pH 8.0, 110mM sodium chloride, 2.2mM potassium chloride, 3mM DTT, 200mM imidazole, 20% glycerol.

Purity

Purified (~44%)

References

1. Trievel, R.C., Shilatifard, A. (2009). Nature Struct. Mol. Biol. 16 (7): 678-80. 2. Wang, Y.Y., et al. (2010). Proc. Natl. Acad. Sci. USA 107(2):815-20. 3. Odho, Z., et al. (2010). J. Biol. Chem. 285(43):32967-76.