West Nile Virus Protease, Fluorimetric, 570 BioAssay(TM) Kit

This kit is optimized to detect West Nile Virus protease activity. It provides enough reagents to perform 100 assays in a 96-well format.\n\nHigh Speed: The entire process only takes about half an hour.\n\nWest Nile virus (WNV) is from the family Flaviviridae3 found in both tropical and\ntemperate regions. The main route of human infection is through the bite of an infected mosquito Infection causes severe neurological disease and fatalities in both human and animal hosts. There is currently no effective vaccine or antiviral drug to protect against WNV infection.4\nWNV contains a single-stranded, positive-sense RNA genome, which encodes three\nstructural proteins [capsid (C), membrane (M), envelope (E)], and seven non-structural proteins\n(NS1, NS2A, NS2B, NS3, NS4A, NS4B, NS5).5,6 West Nile viral NS3 protease is absolutely\nessential (along with viral-encoded cofactor NS2B) for post-translational processing of a viral\npolypeptide precursor in infected host cells. This polypeptide provides the structural and functional viral proteins, and inhibition of its processing could represent a potential treatment for viral infections. Thus, this protease has become one of the key targets for developing anti-WNV drugs.1,7\n\nThe 570 West Nile Virus Protease Assay Kit provides a convenient,\nhomogeneous assay for high throughput screening of West Nile Virus protease NS3 inhibitors. It\nallows for continuous quantification of protease activity using QXL(TM)570/5-TAMRA FRET\npeptide. In the FRET peptide, the fluorescence of 5-TAMRA is quenched by QXL(TM)570. Upon\ncleavage into two separate fragments by WNV protease, the fluorescence of 5-TAMRA is\nrecovered, and can be monitored at excitation/emission = 540 nm/575 nm. The long wavelength fluorescence of 5-TAMRA is less interfered by the autofluorescence of cell components and test compounds.\n\nKit Components:\nComponent A Microtiter Plate 1x100 wells QXLTM570/5-TAMRA, WNV protease substrate, Ex/Em=540nm/575nm 1.5mM DMSO solution, 1x60ul\nComponent B 5-TAMRA, fluorescence reference standard, Ex/Em=540nm/575nm 750uM DMSO solution, 1x10ul\nComponent C Assay Buffer 1x20ml\nComponent D WNV Protease Inhibitor undeca-D-Arg- NH2 1mM DMSO solution, 1 1x0ul\n\nMaterials Required (but not provided):\n

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SPECIFICATIONS

Catalog Number

W1018-70

Size

1Kit

Applications

ELISA

References

1. Shiryaev, SA. et al., Biochem. J. 393, 503 (2006). 2. Chappell, KJ. et al., J. Biol. Chem. 281, 38448 (2006). 3. Hayes, CG. Ann. NY. Acad. Sci. 951, 25 (2001). 4. van der Meulen, KM et al., Arch. Virol. 150, 637 (2005). 5. Brinton, MA. Annu. Rev. Microbiol. 56, 371 (2002). 6. Lanciotti, RS. et al., Science 286, 2333 (1999). 7. Mueller, NH. et al., Int. J. Biochem. Cell Biol. 39, 606 (2007).