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WIBG, Recombinant, Human (Partner of Y14 and Mago, Protein Wibg Homolog, PYM)

Cat no: W3005-60

WIBG, Recombinant, Human (Partner of Y14 and Mago, Protein Wibg Homolog, PYM)

WIBG has been identified as an interacting partner of Mago-Y14. The Mago-Y14 heterodimer is a core component of the EJC(exon junction complex) that is deposited on mRNAs as a consequence of splicing and influences postsplicing mRNA metabolism. This protein is a cytoplasmic RNA-binding protein that is excluded from the nucleus by Crm1. It interacts directly with Mago-Y14 by means of its N-terminal domain.\n\nSource:\nRecombinant corresponding to aa1-204 of human WIBG protein, fused to His-tag at C-terminus, expressed in E.coli.\n\nAA Sequence:\nMEAAGSPAAT ETGKYIASTQ RPDGTWRKQR RVKEGYVPQE EVPVYENKYV KFFKSKPELP PGLSPEATAP VTPSRPEGGE PGLSKTAKRN LKRKEKRRQQ QEKGEAEALS RTLDKVSLEE TAQLPSAPQG SRAAPTAASD QPDSAATTEK AKKIKNLKKK LRQVEELQQR IQAGEVSQPS KEQLEKLARR RALEEELEDL ELGLLEHHHH HH\n\nMolecular Weight: 23.7kD (212aa) confirmed by MALDI-TOF\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for at least 6 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

W3005-60

Size

50ug

Form

Supplied as a liquid in 20mM Tris-HCl, pH 8.0, 10% glycerol, 0.1M sodium chloride.

Purity

>85% (SDS-PAGE).

References

1. Bono F,. et al. (2004) EMBO Rep. 5(3):304-10. 2. Gehring NH,,et al. (2009) Cell. 137(3):536-48.

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