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WNT3 (Proto-oncogene Wnt-3, Proto-oncogene Int-4 Homolog, INT4)

WNT3 (Proto-oncogene Wnt-3, Proto-oncogene Int-4 Homolog, INT4)

Cat no: W3035-55B

Supplier: United States Biological
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Ligand for members of the frizzled family of seven transmembrane receptors. Wnt-3 and Wnt-3a play distinct roles in cell-cell signaling during morphogenesis of the developing neural tube. Applications: Suitable for use in Immunofluorescence, ELISA, Western Blot, Immunohistochemistry and Immunocytochemistry. Other applications not tested. Recommended Dilution: ELISA: 1:32,000 Western Blot: 1-3ug/ml, observed in human brain lysates on ~38kD bands Immunohistochemistry (Paraffin): 3-5ug/ml Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Catalogue number: W3035-55B
Reactivities: Human, Mouse, Rat, Canine
Hosts: Goat
Applications: ELISA, Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Western Blot
Size: 100ug
Form: Supplied as a liquid in Tris saline, 0.02% sodium azide, pH 7.3, 0.5% BSA.
P type: Pab
Purity: Purified by immunoaffinity chromatography.
References: 1. Okamoto M, Inoue K, Iwamura H, Terashima K, Soya H, Asashima M, Kuwabara T. Reduction in paracrine Wnt3 factors during aging causes impaired adult neurogenesis. FASEB J. 2011 Jul 11.
Additional info: Recognizes human WNT3. Species Crossreactivity: Mouse. Species sequence homology: Canine and rat.

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