XAF1 (XIAP-associated Factor 1)

X-linked inhibitor of apoptosis (XIAP)-associated factor 1 (XAF1) is a zinc finger protein that antagonizes the anti-apoptotic activity of XIAP (1,2). XIAP, a member of the inhibitor of apoptosis (IAP) family, inhibits apoptosis by direct inhibition of caspases (3; reviewed in 4). XAF1 is widely expressed in normal tissues, with highest levels in the heart and ovary, but is mostly reduced in cancer lines (1,2). Expression of XAF1 can be induced by interferons via Stat transcriptional activity (5-7). The levels of XAF1 have been shown to be inversely correlated with p53, and p53 is directly responsible for inhibiting XAF1 transcription (8,9). A number of studies have shown that XAF1 can function as a tumor suppressor protein, and decreased levels of XAF1 are found in a variety of different cancers (10-13). Research studies suggest that expression of XAF1 may be a prognostic biomarker for some cancers (14-16).\n\nApplications: \nSuitable for use in Western Blot and Immunoprecipitation. Other applications not tested.\n\nRecommended Dilution:\nWestern Blot: 1:1000. Incubate membrane with diluted antibody in 5% w/v nonfat dry milk, 1X TBS, 0.1% Tween-20 at 4 degrees C with gentle shaking, overnight.\nImmunoprecipitation: 1:50\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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SPECIFICATIONS

Catalog Number

137508

Size

100ul

Applications

IP, WB

Hosts

Rabbit

Reactivities

Hum

Form

Supplied in 10mM sodium HEPES, pH 7.5, 150mM NaCl, 100ug/ml BSA and 50% glycerol.

P Type

Pab

Purity

Purified by Protein A affinity chromatography.

Isotype

IgG

References

1. Fong, W.G. et al. (2000) Genomics 70, 113-22. 2. Liston, P. et al. (2001) Nat Cell Biol 3, 128-33. 3. Deveraux, Q.L. et al. (1997) Nature 388, 300-4. 4. Goyal, L. (2001) Cell 104, 805-8. 5. Leaman, D.W. et al. (2002) J Biol Chem 277, 28504-11. 6. Sun, Y. et al. (2008) Cancer Lett 260, 62-71. 7. Bai, Y. et al. (2008) J Biol Chem 283, 6832-42. 8. Zhang, W. et al. (2010) Int J Oncol 36, 1031-7. 9. Zou, B. et al. (2012) Mol Carcinog 51, 422-32. 10. Byun, D.S. et al. (2003) Cancer Res 63, 7068-75. 11. Ng, K.C. et al. (2004) J Invest Dermatol 123, 1127-34. 12. Ma, T.L. et al. (2005) Chin J Dig Dis 6, 10-4. 13. Zou, B. et al. (2006) Gastroenterology 131, 1835-43. 14. Huang, J. et al. (2010) Cancer Sci 101, 559-67. 15. Chen, Y.B. et al. (2011) Chin Med J (Engl) 124, 3238-43. 16. Wang, Y. et al. (2012) Regul Pept 178, 36-42.

Additional Info

Recognizes endogenous levels of total human XAF1.