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Xanthine Oxidase Fluorometric Assay Kit

Xanthine Oxidase Fluorometric Assay Kit

Cat no: 10010895


Supplier: Cayman Chemical Company
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XO catalyzes the hydroxylation of hypoxanthine to xanthine and then further catalyzes the oxidation of xanthine to uric acid. When oxidizing NADH, XO generates superoxide, a powerful ROS. XO is found predominantly in the liver and intestine. However, levels of circulating XO can increase dramatically with disease. Cayman's XO Assay provides a simple and accurate method for quantifying xanthine oxidase activity. The assay is based on a multistep enzymatic reaction whose end product resorufin, a highly fluorescent compound, can be easily analyzed using an excitation wavelength between 520-550 nm and an emission wavelength between 585-595 nm.
Catalogue number: 10010895
Weight: 0
Form: 96 Well
P type: Assay Kits
Shipping temp: -20
Storage temp: -20
Additional info: Xanthine oxidase (XO) catalyzes the hydroxylation of hypoxanthine to xanthine and then further catalyzes the oxidation of xanthine to uric acid. When oxidizing NADH, XO generates superoxide, a powerful reactive oxygen species. Excessive amounts of superoxide can lead to cell degeneration and death. XO is found predominantly in the liver and intestine. However, levels of circulating XO can increase dramatically with disease. Cayman's Xanthine Oxidase Assay Kit provides a simple and accurate method for quantifying xanthine oxidase activity. The assay is based on a multistep enzymatic reaction whose end product resorufin, a highly fluorescent compound, can be easily analyzed using an excitation wavelength of 520-550 nm and an emission wavelength of 585-595 nm.

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