YAP (65kD Yes-associated Protein, YAP 65, YAP65, YAP-65, Yes Associated Protein 1 65kDa, Yes-associated Protein 1, YAp 1, YAP1, YAP2)

YAP (Yes-Associated Protein) is a founding member of the WW domain family of proteins, transcriptional co-activator and oncogene. Its gene is transcriptionally activated in squamous cell carcinoma cells. As one of several genes of 1q22 amplicon, YAP was recently implicated in liver and breast cancer. Overexpression of YAP gene in MCF10A mammary cell line induces oncogenic transformation.\n\nApplications: \nSuitable for use in Western Blot, Immunoprecipitation and Immunohistochemistry (paraffin-embedded sections). Other applications not tested.\n\nRecommended Dilution:\nOptimal dilutions to be determined by the researcher.\n\nPositive Control: Brain and kidney lysates, and HEK 293 cell lysate\n\nCellular Localization: Nuclear\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. For long-term storage, aliquot and store at -20 degrees C. Aliquots are stable for at least 3 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

Y1200-01D

Size

100ul

Applications

IHC, IP, WB

Hosts

Rabbit

Reactivities

Hum

Form

PBS, 15% glycerol

P Type

Pab

Purity

Purified by immunoaffinity chromatography.

References

1. Sudol, M., et al. Characterization of the Mammilian YAP Gene and Its Role in Defining a Novel Protein Module, the WW Domain. JBC. 14733-14741 (1995).\n2. Komuro, A., et al. WW Domain-containing Protein YAP Associates with WrbB-4 and Acts as a Co-transcriptional Activator for the Carboxyterminal Fragment of ErB4 that Translocates to the Nucleus. JBC. 33334-33341 (2003).\n3. Zender, L., et al. Identification and Validation of Oncogenes in Liver Cancer Using an Integrative Oncogenomic Approach. Cell. 125:1230-3 (2006). [Western blot, Immunohistochemistry]\n4. Dong, G., et al. Molecular Profiling of Transformed and Metastatic Murine Squamous Carcinoma Cells by Differential Display and cDNA Microarray Reveals Altered Expression of Multiple Genes Related to Growth, Apoptosis, Angiogenesis, and the NF-kappaB Signal Pathway. Cancer Res. 61:4797-808 (2001).\n5. Overholtzer, M., et al. Transforming Properties of Yap, a Candidate Oncogene of the Chromosome 11q22 Amplicon. PNAS. 103:12405-10 (2006).

Additional Info

Species Crossreactivity: This antibody reacts with the human protein.