YBOX1, CT (Nuclease-sensitive Element-binding Protein 1, Y-box-binding Protein 1, Y-box Transcription Factor, YB-1, CCAAT-binding, Transcription Factor I Subunit A, CBF-A, Enhancer Factor I Subunit A, EFI-A, DNA-binding Protein B, DBPB, YBX1, NSEP1, YB1)

Y-box-binding protein 1 (YBOX1, YBX1, YB-1) belongs to highly conserved Y-box protein family, a group of multifunctional gene regulators with cold shock domain. Targeting single stranded DNA and RNA, YB-1 regulates both translation and transcription in various genes. The expression of HLA class II genes is regulated by a series of cis-acting elements and trans-acting factors. As a cis-acting element, the Y-Box contains an inverted CCAAT box. An inverse correlation of YB-1 and HLA-DR beta chain mRNA levels suggests that YB-1 is a negative regulatory factor. Over-expression of YB-1 resulted in increased levels of PTP1B. Furthermore, depletion of YB-1 protein, by expression of a specific antisense construct, led to an approximately 70% decrease in expression of PTP1B, but no change in the level of its closest relative, TC-PTP. Data reveal an important role for YB-1 as a regulator of PTP1B expression, and further highlight PTP1B as a critical regulator of insulin- and cytokine-mediated signal transduction. Also, YB-1 has been implicated to activation of genes linked to cancer (e.g. cyclin A/B1, MMP-2, and MDR1). However, it is shown to repress apoptosis related genes (e.g. p53 tumor suppressor). YB-1 promotes separation of DNA strands that contains mismatches or is modified by cisplatin. Thus, YB-1 has been linked to possible role in DNA repair. Applications: Suitable for use in Flow Cytometry, ELISA, Western Blot, Immunoprecipitation, Immunofluorescence and Immunohistochemistry. Other applications not tested. Recommended Dilutions: Western Blot: 1:10,000-1:200,000 Immunohistochemistry (Paraffin): 1:100-1:250 Immunofluorescence (IC): 5mg/ml Flow Cytometry: 1:50-1:100 Optimal dilutions to be determined by the researcher. Positive Controls: HeLa, SW480, A549 and MCF7 cell lysates; kidney tissue Storage and Stability: May be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Manufactured incorporating RabMAb(R) technology under Epitomics US patents, No 5,675,063 and 7,429,487, owned by Abcam.