Yotiao (AKAP9, PRKA9, CG-NAP)

Yotiao is a novel cytoskeletal protein specifically concentrated in the neuromuscular junction and neuronal synapes that interacts with specific splice variants of NMDA Receptor Subunit NR1 in a C1 exon cassette-dependent manner (1). Yotiao has also been shown to physically attach the type I protein phosphatase (PP1) and the adenosine 3',5'-mono-phosphate (cAMP)- dependent protein kinase (PKA) holoenzyme to NMDA receptors to regulate channel activity (2). NMDA receptors are involved with many important functions and dysfunctions of the nervous system, including synapse formation, synaptic plasticity, and excitotoxicity. Yotiao research may lead to a clearer understanding of these neural processes by explaining how NMDA receptors are targeted to particular synapses, how this localization is regulated during development and synaptic activity, and how NMDA receptor activity is transduced into intracellular signals responsible for particular neuronal responses (1). Human tissue distribution studies with yotiao mRNA revealed that yotiao is abundantly present in skeletal muscle and pancreas, with lower expression in heart, placenta, and brain. Yotiao mRNA was undetectable in lung, liver, and kidney (1).\n\nApplications: \nSuitable for use in ELISA and Western Blot. Other applications not tested.\n\nRecommended Dilution:\nELISA: 0.1-1.0ug/ml\nWestern Blot: 1-3ug/ml; Fetal mouse brain~230kD\nAsPC-1 lysates and rat brain homogenates, multiple bands at other molecular weights have also been observed.\nOptimal dilutions to be determined by the researcher.\n\nPositive Controls:\nAsPC-1 cell lysates, rat and fetal mouse brain tissue homogenates. \n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. For long-term storage, aliquot and store at -20 degrees C. Aliquots are stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Prices direct from United States Biological

Quick response times

Exclusive Biosave savings/discounts