ZP4, CT (ZP4, ZPB, Zona pellucida sperm-binding protein 4, Zona pellucida glycoprotein 4, Zona pellucida protein B, Processed zona pellucida sperm-binding protein 4) (FITC)

The zona pellucida is an extracellular matrix that surrounds the oocyte and early embryo. It is composed primarily of three or four glycoproteins with various functions during fertilization and preimplantation development. The nascent protein contains a N-terminal signal peptide sequence, a conserved ZP domain, a consensus furin cleavage site, and a C-terminal transmembrane domain. It is hypothesized that furin cleavage results in release of the mature protein from the plasma membrane for subsequent incorporation into the zona pellucida matrix. However, the requirement for furin cleavage in this process remains controversial based on mouse studies. Previously, this gene has been referred to as ZP1 or ZPB and thought to have similar functions as mouse Zp1. However, a human gene with higher similarity and chromosomal synteny to mouse Zp1 has been assigned the symbol ZP1 and this gene has been assigned the symbol ZP4.\n\nApplications:\nSuitable for use in Western Blot, Immunohistochemistry, ELISA\n\nRecommended Dilution:\nELISA: 1:1,000\nWestern Blot: 1:100-500\nImmunohistochemistry: 1:10-50\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Do not freeze FITC conjugates. Light sensitive. Aliquots are stable for 6 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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SPECIFICATIONS

Catalog Number

044405-FITC

Size

200ul

Applications

ELISA, IHC, WB

Hosts

Rabbit

Reactivities

Hum

Form

Supplied as a liquid in PBS, pH 7.2. Contains ~0.09% sodium azide. Conjugated to FITC.

P Type

Pab

Purity

Purified by Protein A affinity chromatography.

Isotype

IgG

References

Rose, J.E., et al. Mol. Med. 16 (7-8), 247-253 (2010) :\nMcCauley, J.L., et al. Genes Immun. 10(7):624-630(2009)\nNakano, M., et al. Proc. Natl. Acad. Sci. U.S.A. 106(31):12838-12842(2009)\nChoudhury, S., et al. J. Reprod. Immunol. 79(2):137-147(2009)\nChiu, P.C., et al. Biol. Reprod. 79(5):869-877(2008)

Additional Info

Human

Alternative Names

ZP4, ZPB, Zona pellucida sperm-binding protein 4, Zona pellucida glycoprotein 4, Zona pellucida protein B, Processed zona pellucida sperm-binding protein 4