14-3-3 (zeta)(V52) pAb

14-3-3 proteins regulate many cellular processes relevant to cancer biology, notably apoptosis, mitogenic signaling and cell-cycle checkpoints. Seven isoforms comprise this family of signaling intermediates, denoted 14-3-3 (beta), (gamma), E, ?, n, 0 and o. 14-3-3 proteins form dimers that present two binding sites for ligand proteins, thereby bringing together two proteins that may not otherwise associate. These ligands largely share a 14-3-3 consensus binding motif and exhibit serine/threonine phosphorylation. 14-3-3 proteins function in broad regulation of these ligand proteins, by cytoplasmic sequestration, occupation of interaction domains and import/export sequences, prevention of degradation, activation/repression of enzymatic activity and facilitation of protein modification, and thus loss of expression contributes to a vast array of pathogenic cellular activities.

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SPECIFICATIONS

Catalog Number

BS1001

Size

100ug/100ul

Applications

IF, IHC, IP, WB

Hosts

Rabbit

Reactivities

Hum, Mouse, Rat

Form

liquid

Purity

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Antigen

Synthetic peptide, corresponding to amino acids 20-70 of Human 14-3-3 ?.

Species

14-3-3 (zeta)(V52) pAb detects endogenous levels of 14-3-3 protein zeta/delta.

Dilutions

WB: 1:500~1:1000\nIHC: 1:50~1:200\nIP: 1:50~1:200\nIF: 1:50~1:200

Swiss Prot

P63104

Storage Temp

Store at 4 degrees C short term. Aliquot and store at -20 degrees C long term. Avoid freeze-thaw cycles.

Concentration

1ug/ul

Storage Buffer

1 mg/ml in Phosphate buffered saline (PBS) with 0.05% sodium azide, approx. pH 7.2.

Additional Info

For research use only, not for use in diagnostic procedure.

Molecular Weight

~ 28 kDa

Alternative Names

14-3-3 protein zeta/delta; Protein kinase C inhibitor protein 1; KCIP-1; YWHAZ; 14-3-3 protein zeta; 14-3-3 protein delta; 14-3-3 ?; 14-3-3 (delta); 1433Z; KCIP1; KCIP 1