ACC(alpha) (H74) pAb

Acetyl-CoA carboxylase (ACC) is a complex multifunctional enzyme system which catalyzes the carboxylation of acetyl-CoA to malonyl-CoA, the ratelimiting step in fatty acid synthesis. Exercise diminishes the activity of acetyl- CoA carboxylase in human muscle. ACC(alpha) (ACC1) is the rate-limiting enzyme in the biogenesis of long-chain fatty acids, and ACC(beta) (ACC2) may control mitochondrial fatty acid oxidation. These two isoforms of ACC control the amount of fatty acids in the cells. The catalytic function of ACC(alpha) is regulated by phosphorylation (inactive) and dephosphorylation (active) of targeted Serine residues and by allosteric transformation by citrate or palmitoyl-CoA, which serve as the short-term regulatory mechanism of the enzyme. The gene encoding ACC(alpha), which maps to human chromosome 17, encodes the 265 kDa (alpha) form of ACC, which is the major ACC in lipogenic tissues. The catalytic core of ACC(beta) is homologous to that of ACC(alpha) except for an additional peptide of about 150 amino acids at the N-terminus.

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SPECIFICATIONS

Catalog Number

BS1378

Size

100ug/100ul

Applications

IHC, WB

Hosts

Rabbit

Reactivities

Hum, Mouse, Rat

Form

liquid

Purity

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Antigen

Synthetic peptide, corresponding to amino acids 50-100 of Human ACC(alpha).

Species

ACC(alpha) (H74) pAb detects endogenous levels of ACC(alpha) protein.

Dilutions

WB: 1:500~1:1000\nIHC: 1:50~1:200

Swiss Prot

Q13085

Storage Temp

Store at 4 degrees C short term. Aliquot and store at -20 degrees C long term. Avoid freeze-thaw cycles.

Concentration

1ug/ul