Actin, beta

Actin, a ubiquitous eukaryotic protein, is the major component of the cytoskeleton. At least six isoforms are known in mammals. Nonmuscle beta- and gamma-actin, also known as cytoplasmic actin, are predominantly expressed in nonmuscle cells, controlling cell structure and motility (1). alpha-cardiac and alpha-skeletal actin are expressed in striated cardiac and skeletal muscles, respectively; two smooth muscle actins, alpha- and gamma-actin, are found primarily in vascular smooth muscle and enteric smooth muscle, respectively. These actin isoforms regulate contractile potentials for muscle cells (1). Actin exists mainly as a fibrous polymer, F-actin. In response to cytoskeletal reorganizing signals during processes such as cytokinesis, endocytosis, or stress, cofilin promotes fragmentation and depolymerization of F-actin, resulting in an increase in the monomeric globular form, G-actin (2). The Arp2/3 complex stabilizes F-actin fragments and promotes formation of new actin filaments (2). It has been reported that actin is hyperphosphorylated in primary breast tumors (3). Cleavage of actin under apoptotic conditions has been observed in vitro and in cardiac and skeletal muscle (4-6). Actin cleavage by caspase-3 may accelerate ubiquitin/proteosome dependent muscle proteolysis (6).\n\nApplications: \nSuitable for use in Western Blot. Other applications have not been tested. \n\nRecommended Dilutions: \nWestern Blot: 1:1000 \nOptimal dilutions to be determined by the researcher. \n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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SPECIFICATIONS

Catalog Number

A0760-40Q

Size

100ul

Applications

WB

Hosts

Rabbit

Reactivities

Hum, Mouse, Rat, Bov, Dro/Arth, NHP

Form

Supplied as a liquid in 10mM sodium HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, 50% glycerol.

P Type

Pab

Purity

Purified by Protein A and peptide affinity chromatography.

Isotype

IgG

References

Herman, I.M. (1993) Curr. Opin. Cell Biol. 5, 48-55.Condeelis, J. (2001) Trends Cell Biol. 11, 288-293.Lim, Y.P. et al. (2004) Clin. Cancer Res. 10, 3980-3987.Kayalar, C. et al. (1996) Proc. Natl. Acad. Sci. USA. 93, 2234-2238.Communal, C. et al. (2002) Proc. Natl. Acad. Sci. USA. 99, 6252-6256.Du, J. et al. (2004) J. Clin. Invest. 113, 115-123.

Additional Info

Recognizes endogenous levels of human beta-actin at ~45kD. May crossreact with the gamma-actin (cytoplasmic) isoform. Does not crossreact with alpha-skeletal, alpha-cardiac, alpha-vascular smooth or gamma-enteric smooth muscle isoforms. Species crossreactivity: mouse, rat, monkey, mink, hamster, bovine, Drosophila, zebrafish. Species Sequence Homology: porcine, chicken, xenopus, canine.