Actin, Muscle Speci?c

Actin is a major component of the cytoskeleton. Actin can be resolved on the basis of its isoelectric points into three distinctive components: alpha, beta, and gamma in order of increasing isoelectric point. Anti-Muscle-Specific Actin recognizes alpha and gamma isotypes of all muscle groups. Non-muscle cells such as vascular endothelial cells and connective tissues are non-reactive. Also, neoplastic cells of non-muscle-derived tissue such as carcinomas, melanomas, and lymphomas are negative. This antibody is useful in the identification of rhabdoid cellular elements.\n\nApplications:\nSuitable for use in Immunofluorescence and Immunohistochemistry. Other applications have not been tested.\n\nRecommended Dilutions:\nImmunohistochemistry (Formalin/paraffin): 1-2ug/ml for 30 minutes at RT. No special pretreatment is required for immunohistochemical staining of formalin-fixed tissues.\nOptimal dilutions to be determined by the researcher.\n\nPositive Control: \nMuscle or sarcoma \n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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SPECIFICATIONS

Catalog Number

A0760-22A

Size

500ul

Applications

IF, IHC

Hosts

Mouse

Reactivities

Hum, Mouse, Rat, Bov, Can, Prc

Form

Supplied as a liquid in PBS, pH 7.4, 0.2% BSA, 0.09% sodium azide. Also available without BSA and azide., See A0760-22AX.

P Type

Mab

Purity

Purified by Protein G affinity chromatography.

Isotype

IgG1,k

References

1. Tsukada, T., et al., Am. J. Pathol. 126: 51 (1987). 2. Tsukada, T., et. al., Am. J. Pathol. 127: 389 (1987).

Additional Info

Recognizes alpha-smooth muscle, alpha-skeletal and alpha-cardiac (sarcomeric) isoforms of human actin. Reacts with tumors arising from smooth muscle (leiomyosarcomas) and skeletal muscle (rhabdomyosarcomas). Species Crossreactivity: mouse, rat, porcine, equine, bovine and canine.