Actin, Smooth Muscle (alpha-Actin 2, ACTA2)

Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells. In vertebrates three main groups of actin isoforms, alpha, beta and gamma have been identified. The alpha actins are found in muscle tissues and are a major constituent of the contractile apparatus. The beta and gamma actins coexist in most cell types as components of the cytoskeleton and as mediators of internal cell motility.\n\nApplications: \nSuitable for use in Immunofluorescence, ELISA, Western Blot, Immunohistochemistry and Immunocytochemistry. Other applications not tested.\n\nRecommended Dilutions:\nImmunofluorescence: 1:25-1:50 (cells) and 1:100 (vessel sections)\nImmunohistochemistry (FFPE or frozen): 1:50-1:75. Incubate for 30 minutes at RT.\nImmunocytochemistry: 1:75\nOptimal dilutions to be determined by the researcher.\n\nPositive Control: \nLeiomyoma\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Prices direct from United States Biological

Quick response times

Exclusive Biosave savings/discounts

SPECIFICATIONS

Catalog Number

A0759-05D

Size

500ul

Applications

ELISA, ICC, IF, IHC, WB

Hosts

Mouse

Reactivities

Hum

Form

Supplied as a liquid in 20mM Tris-borate, 150mM sodium chloride, 0.09% sodium azide.

P Type

Mab

Purity

Supernatant

Isotype

IgG2a,k

References

1. Skalli O; Alpha-smooth muscle actin, a differentiation marker of smooth muscle cells, is present in microfilamentous bundles of pericytes. J Histochem Cytochem:315-21 (1989). <PUBMED:2918221; 2. Skalli O;; Myofibroblasts from diverse pathologic settings are heterogeneous in their content of actin isoforms and intermediate filament proteins; Lab Invest. 60:275-85 (1989). PUBMED:2644484; 3. Skalli O. et al.; A monoclonal antibody against alpha-smooth muscle actin: a new probe for smooth muscle differentiation; J. Cell Biol. 103:2787-96 (1986). PUBMED:3539945

Additional Info

Recognizes the alpha smooth muscle isoform of human actin, smooth muscle cells of blood vessels and parenchymal tissue of intestine, testis, and ovary. Does not react with actin from fribroblasts, striated muscle or myocardium.

Alternative Names

ACTSA, ACTVS, ACTSA, alpha Smooth Muscle Actin