Actinin-(alpha)2/3 (Q58) pAb

The spectrin gene family encodes a diverse group of cytoskeletal proteins that include spectrins, dystrophins and (alpha)-actinins. There are four tissue-specific (alpha)-actinins, namely (alpha)-actinin-1, (alpha)-actinin-2, (alpha)-actinin-3 and (alpha)-actinin-4, which are localized to muscle and non-muscle cells, including skeletal, cardiac and smooth muscle cells, as well as within the cytoskeleton. Each (alpha)-actinin protein contains one Actin-binding domain, two calponin-homology domains, two EF-hand domains and four spectrin repeats, through which they function as bundling proteins that can cross-link F-Actin, thus anchoring Actin to a variety of intracellular structures. Defects in the gene encoding (alpha)-actinin-4 are the cause of focal segmental glomerulosclerosis 1 (FSGS1), a common renal lesion characterized by decreasing kidney function and, ultimately, renal failure.

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SPECIFICATIONS

Catalog Number

BS1984

Size

100ug/100ul

Applications

IF, IHC, WB

Hosts

Rabbit

Reactivities

Hum, Mouse, Rat

Form

liquid

Purity

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Antigen

Synthetic peptide, corresponding to amino acids 30-70 of Human Actinin-(alpha)2.

Species

Actinin-(alpha)2/3 (Q58) pAb detects endogenous levels of actinin-(alpha)2 and actinin-(alpha)3 protein.

Dilutions

WB: 1:500~1:1000\nIHC: 1:50~1:200\nIF: 1:50~1:200

Swiss Prot

P35609/Q08043

Storage Temp

Store at 4 degrees C short term. Aliquot and store at -20 degrees C long term. Avoid freeze-thaw cycles.

Concentration

1ug/ul

Storage Buffer

1 mg/ml in Phosphate buffered saline (PBS) with 0.05% sodium azide, approx. pH 7.2.

Additional Info

For research use only, not for use in diagnostic procedure.

Molecular Weight

~ 103 kDa

Alternative Names

Alpha-actinin-2; Alpha-actinin skeletal muscle isoform 2; F-actin cross-linking protein; ACTN2; (alpha)-Actinin2; Actinin (alpha)2; Actinin(alpha)2; (alpha)Actinin-2; Alpha-actinin-3; Alpha-actinin skeletal muscle isoform 3; ACTN3; (alpha)-Actinin3; Actinin (alpha)3; Actinin(alpha)3; (alpha)Actinin-3; Actinin-(alpha)3; (alpha)-actinin-2; (alpha)-actinin-3