Suitable for use in nucleic acid inhibition. Actinomycin D inhibits the proliferation of cells in a cell-cycle nonspecific way by forming a stable complex with DNA (via deoxyguanosine residues), thus inhibiting DNA-primed RNA synthesis. It is used in cell culture as a selection agent.
Chemical Name: 2-Amino-(N,N)-1-bis(hexadecahydro-6�,13-diisopropyl-2,5,9-trimethyl-1,4,7,11,14-pentao�xo-1H-pyrrolo[2,1]-[1,4,7,10,13]oxatetraazacyclohexadecin-10-yl)-4,6-dimethyl-3-ox�o-3H-phenoxazine-1,9-dicarboxamide
Purity: (same/more than)95%
Appearance: Orange to red-brown crystaline powder
Identity: Conforms to USP reference
Potency: (same/more than)900u/mg
Specific Rotation:-292 to -317
Heavy Metals: <0.001%
Loss on Drying: (same/less than) 5%
RNase:None Detected
DNase: None Detected
Protease: None Detected
Residue on Ignition: (same/less than)0.1%
Endotoxin: (same/less than)100EU/mg
Working concentration: 0.01 to 2ug/ml
Solublility: Dimethyl sulfoxide (DMSO) 1mg/ml
Light and moisture sensitive.