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Activation-induced Cytidine Deaminase (AICDA, AID, ARP2, CDA2, HIGM2)

Activation-induced Cytidine Deaminase (AICDA, AID, ARP2, CDA2, HIGM2)

Cat no: A0855-75J

Supplier: United States Biological
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Activation-induced cytidine deaminase (AID) was initially discovered as a homolog of the apolipoprotein B RNAediting cytidine deaminase 1 (APOBEC1) that showed cytidine deaminase properties in stimulated B cell lines (1). It is necessary for somatic hypermutation and class switch recombination in B cells (2), but inappropriate or dysregulated expression AID is often found in tumors and B cell neoplasms (2,3). Although it is structurally and functionally similar to the APOBEC proteins (reviewed in 4), it appears unlikely that AID deaminates dC to dU residues in HIV cDNA as does APOBEC3G (5). Applications: Suitable for use in ELISA, Western Blot, Immunocytochemistry. Other applications not tested. Recommended Dilution: Western Blot: 1-2ug/ml. See Control Peptide, A0855-75J1. Immunocytochemistry: 5-10ug/ml Optimal dilutions to be determined by the researcher. Positive Control: Whole cell lysate from Ramos cells Storage and Stability: May be stored at 4 degrees C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and store at -20 degrees C. Aliquots are stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Catalogue number: A0855-75J
Reactivities: Human, Mouse
Hosts: Rabbit
Applications: ELISA, Immunocytochemistry, Western Blot
Size: 100ug
Form: Supplied as a liquid in PBS, pH 7.2, 0.02% sodium azide.
P type: Pab
Isotype: IgG
Purity: Purified by immunoaffinity chromatography.
Alternative names: EC=3.5.4.5
References: 1. Muramatsu M, Sankaranand VS, Anant S, et al. Specific expression of activation-induced cytidine deaminase (AID), a novel member of the RNA -editing deaminase family in germinal center B cells. J. Biol. Chem. 1999; 274:18470-6. 2. Muramatsu M, Kinoshita K, Fagarasan S, et al. Class switch recombination and hypermutation require activation-induced cytidine deaminase (AID), a potential RNA editing enzyme. Cell 2000; 102:553-63. 3. Greeve J, Philipsen A, Krause K, et al. Expression of activation-induced cytidine deaminase in human B-cell Hodgkin lymphomas. Blood 2003; 101:3574-80. 4. Cascalho M. Advantages and disadvantages of cytidine deamination. J. Immunol. 2004; 172:6513-8. 5. Sheehy AM, Gaddis NC, Choi JD, et al. Isolation of a human gene that inhibits HIV -1 infection and is suppressed by the viral Vif protein. Nature 2002; 418:646-50. (06-01D)
Additional info: Recognizes human Activation-induced Cytidine Deaminase (AICDA, AID, ARP2, CDA2, HIGM2). Species Crossreactivity: mouse.

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