Members of ADAM family are cell surface proteins with a unique structure possessing both potential adhesion and protease domains. The extracellular region of ADAM8 shows significant amino acid sequence homology to hemorrhagic snake venom proteins, including the metalloprotease and disintegrin domains. The expression of ADAM8 is upregulated by retinoic acid and vitamin D3. ADAM8 is involved in protein processing at the cell surface and is also thought to act as a leukocyte differentiation marker.
Applications:
Suitable for use in Flow Cytometry, Direct ELISA, Western Blot, and Immunoprecipitation. Other applications not tested.
Recommended Dilutions:
Flow Cytometry: 25ug/ml. 10ul of the diluted solution labels 1-2.5x10e5 cells in a total reaction volume <200ul. The binding of unlabeled monoclonal antibodies may be visualized by adding 10ul of a 25ug/ml stock solution of a secondary developing reagent such as goat anti-mouse IgG conjugated to a fluorochrome.
Direct ELISA: 1ug/ml detects human ADAM8 (ecto) coated on 96 well plates. The detection limit is ~1ng/well.
Western Blot: 1-2ug/ml. Using a colorimetric detection system, the detection limit is ~20ng/lane under non-reducing conditions.
Immunoprecipitation: 25ug/ml immunoprecipitates ADAM8 from conditioned cell culture media. The immunoprecipitated ADAM8 can be detected in western blots using an affinity-purified goat polyclonal antibody (Catalog #A0858-98B or A0858-98E).
Optimal dilutions to be determined by the researcher.
Storage and Stability:
Lyophilized powder may be stored at -20 degrees C. Stable for 12 months at -20 degrees C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Reconstituted product is stable for 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.�
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