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ADD1, phosphorylated (Ser726) (Alpha-adducin, Erythrocyte Adducin Subunit alpha, ADDA)

ADD1, phosphorylated (Ser726) (Alpha-adducin, Erythrocyte Adducin Subunit alpha, ADDA)

Cat no: A0859-55Q

Supplier: United States Biological
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ADD1 is a ubiquitously expressed membrane-skeletal protein localized at spectrin-actin junctions that binds calmodulin and is an in vivo substrate for protein kinase C (PKC) and Rho-associated kinase. ADD1 is a tetramer comprised of either f/g or f/n heterodimers. ADD1 subunits are related in sequence and all contain an N-terminal globular head domain, a neck domain and a C-terminal protease-sensitive tail domain. The tail domains of all ADD1 subunits end with a highly conserved 22-residue myristoylated alanine-rich C kinase substrate (MARCKS)-related domain that has homology to MARCKS protein. It caps the fast-growing ends of actin filaments and also preferentially recruits spectrin to the ends of filaments. Recent observations suggest a role for the protein in cell motility, and as a target for regulation by Rho-dependent and Ca2+-dependent pathways. Human ADD1 gene is mapped to chromosomal region 4p16.3. Applications: Suitable for use in Western Blot. Other applications not tested. Recommended Dilution: Western Blot: 1:50-1:100 Optimal dilutions to be determined by the researcher. Positive Control: HT-29 cells Storage and Stability: May be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Catalogue number: A0859-55Q
Reactivities: Human, Mouse, Rat
Hosts: Rabbit
Applications: Western Blot
Size: 50ug
Form: Supplied as a liquid in PBS (without Mg2+ and Ca2+), pH 7.4, 150mM sodium chloride, 0.02% sodium azide, 50% glycerol.
P type: Pab
Isotype: IgG
Purity: Purified by immunoaffinity chromatography.
References: 1. Matsuoka. Y. et al. Cellular and Molecular Life Sciences. 57:884-895 (2004).
Additional info: Recognizes human ADD1, phosphorylated at Ser726. Species Crossreactivity: mouse and rat.

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