Adenosine A2a Receptor (A2aR)

Adenosine receptors (ARs) are members of the 7-transmembrane domain G-protein-coupled receptor superfamily. Structural, biochemical and pharmacological analyses of the AR genes and protein has led to the discovery of four distinct AR subtypes (A1, A2a, A2b, A3). Activation of ARs mediates several receptor subtype-specific physiological processes that include cardiac rate, smooth muscle tone, platelet aggregation, inflammation, cell growth and death, and neurotransmission. The A2aAR is a glycoprotein that can activate adenylyl cyclase via Gs interactions. Stimulation of A2aAR and the resultant accumulation of cAMP inhibits platelet aggregation and in certain vascular beds is associated with vasodilation and drop in blood pressure. A2aAR is reported to interact with D2 dopamine receptor sites in the brain where they co-express (striatum, olfactory tubercle and nucleus accumbens). Other tissues including heart, kidney, and lung also express A2aAR.\n\nApplications: \nSuitable for use in Western Blot, Immunoprecipitation and Immunohistochemistry. Other applications not tested.\n\nRecommended Dilutions:\nWestern Blot: 1:1000 detects an ~45kD protein from canine striatum representing A2aAR.\nImmunohistochemistry (paraffin-embedded sections): 1:100. Staining of A2aAR in human hippocampus yeilds a pattern consistent with plasma memrane staining.\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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SPECIFICATIONS

Catalog Number

A0869-56A1

Size

100ul

Applications

IHC, IP, WB

Hosts

Rabbit

Reactivities

Hum, Can

Form

Supplied as a liquid in PBS, 1mg/ml BSA, 0.05% sodium azide.

P Type

Pab

Purity

Purified by affinity chromatography.

Isotype

IgG

References

Olah ME & Stiles GL Adenosine receptor subtypes: characterization and therapeutic regulation. Annu Rev Pharmacol Toxicol 35:581-606 (1995). Palmer TM & Stiles GL Adenosine receptors. Neuropharmacology 34:683-94 (1995).

Additional Info

Recognizes canine adenosine receptor A2a at ~45kD. Does not detect other AR subtypes. Species Crossreactivity: human

Alternative Names

ADORA2A, HGNC:263, RDC8, hA2aR, Adenosine Receptor Subtype A2a