Adiponectin (Acrp30, Adipocyte Complement-related Protein of 30kD, AdipoQ, apM1, Gelatin Binding Protein 28, GPB28)

Adiponectin, also referred to as Acrp30, AdipoQ and GBP-28, is a recently discovered 244 amino acid protein, the product of the apM1 gene which is physiologically active and specifically and highly expressed in adipose cells (adipokine).\nThe protein belongs to the soluble defence collagen superfamily. It has a collagen-like domain which is structurally homologous with collagen VIII and X and complement factor C1q-like globular domain. Adiponectin forms homotrimers, which are the building blocks for higher-order complexes found circulating in serum. A reduction in adiponectin serum levels is accompanied by insulin resistance states such as obesity and type 2 diabetes mellitus. It is also reported in patients with coronary artery disease. Increased adiponectin levels are associated with type 1 diabetes mellitus, anorexia nervosa and chronic renal failure.\nAdiponectin has been shown to increase insulin sensitivity and decrease plasma glucose by increasing tissue fat oxidation. It inhibits the inflammatory processes of atherosclerosis suppressing the expression of adhesion and cytokine molecules in vascular endothelial cells and macrophages, respectively.\n\nApplications:\nSuitable for use in ELISA and Western Blot. Other applications not tested.\n\nRecommended Dilution:\nIndirect ELISA: >1:100,000 using 2.5ng of antigen coated per well\nWestern Blot: 1:1000\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nLyophilized powder may be stored at -20 degrees C. Stable for 12 months at -20 degrees C. Reconstitute with sterile ddH2O or PBS. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Reconstituted product is stable for 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

A0891-01

Size

100ug

Applications

ELISA, WB

Hosts

Rabbit

Reactivities

Hum

Form

Supplied as a lyophilized powder in PBS, pH 7.2

P Type

Pab

Purity

Purified by immunoaffinity chromatography.

Isotype

IgG

References

1. Scherer PE, Williams S, Fogliano M, Baldini G, Lodish HF. A novel serum protein similar toC1q, produced exclusively in adipocytes. J Biol Chem. 1995, 270, 26746-26749. 2. Maeda K, Okubo K, Shimomura I, Funahashi T, Matsuzawa Y, Matsubara K. cDNA cloningandexpression of a novel adipose specific collagen-like factor, apM1 (adipose most abundantgene transcript 1). Biochem Biophys Res Commun. 1996, 221, 286-289 3. Saito K, Tobe T, Minoshima S, Asakawa S, Sumiya J, Yoda M, Nakano Y, Shimizu N, Tomita M. Organization of the gene for gelatin-binding protein (GBP28). Gene. 1999, 229, 67-73.

Additional Info

Recombinant human adiponectin is 100% homologous with the human serum adiponectin.