Adrenomedullin (ADM) is a secreted, monomeric, 6kD member of the adrenomedullin family of molecules. It is widely expressed, being found in smooth muscle cells, endothelium, adrenal medulla chromaffin cells, fibroblasts and keratinocytes. Adrenomedullin has multiple functions including vasodilation, the maintenance of vascular integrity, and the suppression of inflammatory mediator secretion. The human Adrenomedullin preproprecursor is 185aa in length. It contains a 21aa signal sequence, a processed 20aa peptide termed PAMP aa22-41, an N-terminal propeptide aa45-92, the Adrenomedullin precursor (amidation is required for maturation) aa95-147, and a C-terminal propeptide aa148-185. The Adrenomedullin precursor with a terminal Gly147 circulates naturally with bioactive, mature amidated Adrenomedullin aa95-146. Depending upon the tissue, truncated forms of Adrenomedullin likely also occur, including variants spanning aa120-146,128-146. Over aa22-147, the human Adrenomedullin proprecursor shares 70aa identity with mouse Adrenomedullin proprecursor.
Applications:
Suitable for use in Immunohistochemistry. Other applications not tested.
Recommended Dilution:
Immunohistochemistry: 8-25ug/ml using Immersion fixed paraffin-embedded sections of human heart.
Optimal dilutions to be determined by the researcher.
Storage and Stability:
Lyophilized powder may be stored at -20 degrees C. Stable for 12 months at -20 degrees C. Reconstitute with PBS. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Reconstituted product is stable for 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
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