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Aequorin

Cat no: A0921

Aequorin

Aequorin is a photoprotein originally characterized in the luminescent jellyfish Aequorea victoria. The jellyfish is capable of producing flashes of blue light, which in turn is transduced to green by the famous green fluorescent protein. The intracellular signal used to elicit this flash in vivo is an increase in intracellular calcium. As the dynamic range of calcium signaling concentrations in jellyfish is very similar to those in plants and animals generally (5-50nM), this means that aequorin is a useful calcium indicator. Aequorin was first used by direct extraction from jellyfish and microinjection, but over the last decade, recombinant aequorin has become available, and it has also been used as a transgene, originally in plants.\n\nApplications: \nSuitable for use in ELISA and Western Blot. Other applications not tested.\n\nRecommended Dilutions:\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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SPECIFICATIONS

Catalog Number

A0921

Size

100ul

Applications

ELISA, WB

Hosts

Mouse

Form

Supplied as a liquid.

P Type

Mab

Purity

Ascites

Isotype

IgG

Additional Info

Recognizes aequorin

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