Akt3 (RAC-PK Gamma)

Akt, protein kinase B (PKB), is a serine/threonine kinase which is involved in many cellular signaling pathways and acts as a transducer of many functions initiated by growth factor receptors that activat phosphtidylinositol 3 kinase (PI 3 kinase). The major activity of Akt/PKB is to mediate cell survival. Akt/PKB is also believed to be a critical factor in the genesis of cancer as the tumor suppressor PTEN was found to antagonise PI3 kinase and Akt/PKB kinase activity. There are 3 known isoforms of this enzyme in mammalian cells (1/alpha, 2/beta and 3/gamma). IGF1 leads to the activation of AKT3, which may play a role in regulating cell survival. It is capable of phosphorylating several known proteins.\n\nApplications: \nSuitable for use in ELISA and Western Blot. Other applications not tested.\n\nRecommended Dilution:\nELISA: 1:16,000.\nWestern Blot: 1-3ug/ml. A 50-55kD band observed in Human Hepatoblastoma HepG2 lysates (calculated MW of 55.8kD according to NP_005456 and 54.0kD according to NP_859029).\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\n\nMay be stored at 4 degrees C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and store at -20 degrees C. Aliquots are stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

A1125-30K

Size

100ug

Applications

ELISA, WB

Hosts

Goat

Reactivities

Hum, Mouse, Rat

Form

Supplied as a liquid in Tris-saline, pH 7.2. 0.5% BSA, 0.02% sodium azide.

P Type

Pab

Purity

Purified by immunoaffinity chromatography.

Isotype

IgG

References

Zinda MJ et al. AKT-1, -2, and -3 are expressed in both normal and tumor tissues of the lung, breast, prostate, and colon. Clin Cancer Res 7:2475-9 (2001). PubMed: 11489829

Additional Info

Species Crossreactivity: Reacts with Human. Predicted to react with Mouse (100% identity with immunogen) and Rat (100% identity with immunogen) due to sequence homology.

Alternative Names

DKFZP434N0250, PKBG, Protein Kinase B Gamma, RAC Gamma Serine/Threonine Protein Kinase, RACPK Gamma, Serine Threonine Protein Kinase AKT3, STK2, VAKT Murine Thymoma Viral Oncogene Homolog 3