Akt3 (RAC-PK Gamma)

Akt, protein kinase B (PKB), is a serine/threonine kinase which is involved in many cellular signaling pathways and acts as a transducer of many functions initiated by growth factor receptors that activat phosphtidylinositol 3-kinase (PI 3-kinase). The major activity of Akt/PKB is to mediate cell survival. Akt/PKB is also believed to be a critical factor in the genesis of cancer as the tumor suppressor PTEN was found to antagonise PI-3 kinase and Akt/PKB kinase activity. There are 3 known isoforms of this enzyme in mammalian cells (1/alpha, 2/beta and 3/gamma). Akt1, 2 and 3 contain a key regulatory serine phosphorylation site in the carboxy-terminal region of the protein. Akt/PKB phosphorylation is critical for it activity.\n\nCellular Localization: Cytoplasmic and membrane-associated after cell stimulation leading to its translocation.\n\nApplications: \nSuitable for use in Western Blot. Other applications not tested.\n\nRecommended Dilution:\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot Store at -20 degrees C. Aliquots are stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

A1125-30L

Size

100ug

Applications

WB

Hosts

Mouse

Reactivities

Hum, Mouse, Rat

Form

PBS containing 0.02% sodium azide, 0.1 mg/ml BSA.

P Type

Mab

Purity

Purified by Protein G affinity chromatography.

Isotype

IgG1

References

Ferrigno P & Silver PA Regulated nuclear localization of stress-responsive factors: how the nuclear trafficking of protein kinases and transcription factors contributes to cell survival. Oncogene 18:6129-34 (1999). Kandel ES & Hay N The regulation and activities of the multifunctional serine/threonine kinase Akt/PKB. Exp Cell Res 253:210-29 (1999). Franke TF et al. PI3K: downstream AKTion blocks apoptosis. Cell 88:435-7 (1997). Alessi DR et al. Mechanism of activation of protein kinase B by insulin and IGF-1. EMBO J 15:6541-51 (1996).

Additional Info

A 50kD band is observed. Species Crossreactivity: Crossreacts with Human, Mouse and Rat.

Alternative Names

DKFZP434N0250, PKBG, Protein Kinase B Gamma, RAC Gamma Serine/Threonine Protein Kinase, RACPK Gamma, Serine Threonine Protein Kinase AKT3, STK2, VAKT Murine Thymoma Viral Oncogene Homolog 3