AMPK(alpha)1 pAb

AMPK is a heterotrimeric complex comprising a catalytic (alpha) subunit and regulatory (beta) and (gamma) subunits. It protects cells from stresses that cause ATP depletion by switching off ATP-consuming biosynthetic pathways. AMPK is activated by high AMP and low ATP through a mechanism involving allosteric regulation, promotion of phosphorylation by an upstream protein kinase known as AMPK kinase, and inhibition of dephosphorylation. Activated AMPK can phosphorylate and regulate in vivo hydroxymethylglutaryl-CoA reductase and acetyl-CoA carboxylase, which are key regulatory enzymes of sterol synthesis and fatty acid synthesis, respectively. The human AMPK(alpha)1 and AMPK(alpha)2 genes encode 548 amino acid and 552 amino acid proteins, respectively. Human AMPK(beta)1 encodes a 271 amino acid protein and human AMPK(beta)2 encodes a 272 amino acid protein. The human AMPK(gamma)1 gene encodes a 331 amino acid protein. Human AMPK(gamma)2 and AMPK(gamma)3, which are 569 and 492 amino acid proteins, respectively, contain unique N-terminal domains and may participate directly in the binding of AMP within the AMPK complex.

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SPECIFICATIONS

Catalog Number

BS6271

Size

100ug/100ul

Applications

IHC, WB

Hosts

Rabbit

Reactivities

Hum, Mouse, Rat

Form

liquid

Purity

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Antigen

Recombinant full length Human AMPK(alpha)1.

Species

AMPK(alpha)1 pAb detects endogenous levels of AMPK(alpha)1 protein.

Dilutions

WB: 1:500~1:2000\nIHC: 1:50~1:200

Swiss Prot

Q13131

Storage Temp

Store at 4 degrees C short term. Aliquot and store at -20 degrees C long term. Avoid freeze-thaw cycles.

Concentration

1ug/ul

Storage Buffer

1 mg/ml in Phosphate buffered saline (PBS) with 0.05% sodium azide, approx. pH 7.2.

Additional Info

For research use only, not for use in diagnostic procedure.

Molecular Weight

~ 63 kDa

Alternative Names

5'-AMP-activated protein kinase catalytic subunit alpha-1; AMPK subunit alpha-1; Acetyl-CoA carboxylase kinase; ACACA kinase; Hydroxymethylglutaryl-CoA reductase kinase; HMGCR kinase; au-protein kinase PRKAA1; PRKAA1; AMPK1