Anaplastic Lymphoma Kinase (ALK, p80)

Anaplastic large-cell lymphoma (Ki-1 lymphoma) is one of the more commonly occurring forms of T cell lymphoma in humans. The majority of these tumors (> 60%) are associated with a t (2; 5) (p23; q35) chromosomal translocation, which results in an abnormal fusion gene involving the ALK gene and the nucleophosmin (NPM) gene (located on chromosome 5q35), which codes for a ubiquitously expressed nucleolar phosphoprotein that functions in transporting components of ribosomes between the cytoplasm and nucleolus during the final stages of ribosome assembly. Transcription of this abnormal NPM-ALK fusion gene results in the production of an abnormal protein, called p80 that functions as a protein tyrosine kinase and as a potent oncogene. The protein functions through Ras-dependent pathways, which is evident from a direct physical association between the p80 and phosphotyrosine binding Src homology adaptor proteins Grb2, Shc, and IRS-i.\n\nApplications:\nSuitable for use in Immunohistochemistry. Other applications not tested.\n\nRecommended Dilution:\nImmunohistochemistry: Paraffin; recommended to incubate for 60 min at RT.\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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SPECIFICATIONS

Catalog Number

A2277-02D

Size

500ul

Applications

IHC

Hosts

Mouse

Reactivities

Hum, Mouse

Form

Supplied as a liquid.

P Type

Mab

Purity

Supernatant

Isotype

IgG1

References

1. Downing, et al., Blood 85: 3416 (1995). 2. Pittaluga, et al., Am. J. Pathol. 151: 343 (1997). 3. Pulford, et al., Blood 89: 1394 (1997). 4. Wellmann, et al., The FASEB Journal 11: 965 (1997).

Additional Info

Recognizes human ALK. Species Crossreactivity: mouse.