Anaplastic large-cell lymphoma (Ki-1 lymphoma) is one of the more commonly occurring forms of T cell lymphoma in humans. The majority of these tumors (> 60%) are associated with a t (2; 5) (p23; q35) chromosomal translocation, which results in an abnormal fusion gene involving the ALK gene and the nucleophosmin (NPM) gene (located on chromosome 5q35), which codes for a ubiquitously expressed nucleolar phosphoprotein that functions in transporting components of ribosomes between the cytoplasm and nucleolus during the final stages of ribosome assembly. Transcription of this abnormal NPM-ALK fusion gene results in the production of an abnormal protein, called p80 that functions as a protein tyrosine kinase and as a potent oncogene. The protein functions through Ras-dependent pathways, which is evident from a direct physical association between the p80 and phosphotyrosine binding Src homology adaptor proteins Grb2, Shc, and IRS-i.
Applications:
Suitable for use in Immunohistochemistry. Other applications not tested.
Recommended Dilution:
Immunohistochemistry: Paraffin; recommended to incubate for 60 min at RT.
Optimal dilutions to be determined by the researcher.
Storage and Stability:
May be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
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