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ATP-Citrate Lyase (ACLY, ATP Citrate (pro-S) Lyase, ATP Citrate Synthase, Citrate Cleavage Enzyme, CLATP, EC 2.3.3.8)

Cat no: 029975

ATP-Citrate Lyase (ACLY, ATP Citrate (pro-S) Lyase, ATP Citrate Synthase, Citrate Cleavage Enzyme, CLATP, EC 2.3.3.8)

ATP citrate lyase is the primary enzyme responsible for the synthesis of cytosolic acetyl-CoA in many tissues. The enzyme is a tetramer (relative molecular weight approximately 440,000) of apparently identical subunits. It catalyzes the formation of acetyl-CoA and oxaloacetate from citrate and CoA with a concomitant hydrolysis of ATP to ADP and phosphate. The product, acetyl-CoA, serves several important biosynthetic pathways, including lipogenesis and cholesterogenesis. In nervous tissue, ATP citrate-lyase may be involved in the biosynthesis of acetylcholine.\n\nApplications:\nWestern Blot: 1:500-1:2000\nImmunohistochemistry: 1:200-1:1000\nImmunocytochemistry: 1:50\nFlow Cytometry: 1:200-1:400\nELISA: 1:10000\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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SPECIFICATIONS

Catalog Number

029975

Size

100ug

Applications

ELISA, FC, ICC, IHC, WB

Hosts

Mouse

Reactivities

Hum, Mouse, NHP

Form

Purified antibody in PBS with 0.05% sodium azide and 0.5% protein stabilizer

P Type

Mab

Purity

Purified

Isotype

IgG1

References

1.J Biol Chem. 2010 Oct 15;285(42):32606-15. 2.Int J Cancer. 2010 May 15;126(10):2282-95.

Additional Info

Human, Mouse, Monkey,

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SUPPLIER INFO

Applications

ELISA

Reactivities

Hum

More info

Applications

IF

Hosts

Mouse

More info

Applications

ELISA, WB

Hosts

Mouse

Reactivities

Hum

More info

Applications

ELISA, FC, WB

Hosts

Mouse

Reactivities

Hum

More info

Applications

ELISA, FC, IHC, WB

Hosts

Mouse

More info

Applications

IHC, WB

Hosts

Rabbit

Reactivities

Hum

More info

Applications

ELISA, WB

Hosts

Rabbit

Reactivities

Hum

More info

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