ATPase alpha 1, Na, K

Na+/K+ ATPase a-1 is a human gene and functions to maintain Na+ and K+ across the cell membrane which is important for osmoregulation. It is a component of (Na+/K+) ATPase, a transporter that catalyzes the hydrolysis of ATP and transports sodium and potassium ions across the plasma membrane. It also partners with Src and other signaling proteins. Binding of ouabain and other cardiotonic steroids to the (Na+/K+) ATPase activates Src, resulting in the assembly and activation of multiple signaling cascades. Binding of ouabain also induces a positive inotropic effect. Diseases associated with ATPase are Thyrotoxic periodic paralysis and hyperthyroidism.\n\nApplications:\nSuitable for use in Immunoprecipitation and Immunohistochemistry. Other applications have not been tested.\n\nRecommended Dilutions:\nImmunoprecipitation: 10ug immunoprecipates ATPase from 500ug of rat brain microsomal lysates.\nImmunohistochemistry: 1:500\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability: \nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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SPECIFICATIONS

Catalog Number

A4000-53

Size

50ug

Applications

IHC, IP

Hosts

Rabbit

Reactivities

Hum, Rat

Form

Supplied as a liquid in 0.1M Tris-glycine, pH 7.4, 0.15M sodium chloride, 0.05% sodium azide.

P Type

Pab

Purity

Purified by Protein A affinity chromatography.

Isotype

IgG

References

1. Shyjan, A.W. and Levenson, R., Biochemistry 28: 4531-4535, 1989. 2. Jorgenson, P.L., Biochim. Biophys. Acta 356: 36-52, 1974.

Additional Info

Recognizes rat ATPase a-1 subunit isoform at Mr of 110kD. Species Crossreactivity: human. Species Sequence Homology: mouse, 99%; porcine, rabbit, monkey, 96%.