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Aurora A (Serine/Threonine-protein Kinase 6, Aurora Kinase A,  Aurora/IPL1-related Kinase 1, Aurora-related Kinase 1, ARK-1, hARK1, Breast Tumor-amplified Kinase, AURKA, ARK1, AURA, BTAK, STK6, AIK)

Cat no: A4190

Aurora A (Serine/Threonine-protein Kinase 6, Aurora Kinase A, Aurora/IPL1-related Kinase 1, Aurora-related Kinase 1, ARK-1, hARK1, Breast Tumor-amplified Kinase, AURKA, ARK1, AURA, BTAK, STK6, AIK)

Aurora A/AIK, a cell cycle-regulated serine/threonine protein kinase, is overexpressed in many tumor cell lines. Phosphorylation of threonine 288, which is within the activation loop of the kinase, results in a significant increase in its enzymatic activity. Aurora A/AIK is regulated by proteasome-dependent degradation, and phosphorylation on Thr288 may target it for degradation during mitosis. Thus, phosphorylation of Aurora A/AIK at Thr288 is important for both kinase activity and stability.\n\nApplications: \nSuitable for use in ELISA, and Western Blot. Other applications not tested.\n\nRecommended Dilution:\nWestern Blot: 1:1,000. Incubate membrane with diluted antibody in 5% BSA, 1X TBS, 0.1% Tween-20 at 4 degrees C with gentle shaking, overnight.\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability: \nMay be stored at 4 degrees C for short-term only. For long-term storage, store at -20 degrees C. Aliquots are stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

A4190

Size

100ul

Applications

ELISA, WB

Hosts

Rabbit

Reactivities

Hum, NHP

Form

Supplied as liquid in 10mM HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, 50% glycerol. No preservative added.

P Type

Pab

Purity

Purified by Protein A and Peptide Immunoaffinity chromatography.

Isotype

IgG

References

1. Bischoff, J.R. et al. (1998) EMBO J. 17, 3052

Additional Info

Detects endogenous levels of human Aurora A/AIK proteins. This antibody may crossreact with AIK3. Species crossreactivity: monkey.

Read more on Supplier website

Applications

ELISA

Reactivities

Hum

More info

Applications

IF

Hosts

Mouse

More info

Applications

ELISA, WB

Hosts

Mouse

Reactivities

Hum

More info

Applications

ELISA, FC, WB

Hosts

Mouse

Reactivities

Hum

More info

Applications

ELISA, FC, IHC, WB

Hosts

Mouse

More info

Applications

IHC, WB

Hosts

Rabbit

Reactivities

Hum

More info

Applications

ELISA, WB

Hosts

Rabbit

Reactivities

Hum

More info
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