Autophagy Protein, Antibody Sampler Set, BioAssay(TM)

Autophagy, the process of bulk degradation of cellular proteins through an autophagosomic-lysosomal pathway is important for normal growth control and may be defective in tumor cells. It is involved in the preservation of cellular nutrients under starvation conditions as well as the normal turnover of cytosolic components. In mammalian cells, APG7 is essential for autophagy conjugation systems, autophagosome formation, starvation-induced bulk degradation of proteins and organelles. Beclin-1, a coiled- coil Bcl-2-interacting protein homologous to the yeast autophagy gene apg6 is a mammalian autophagy gene that can inhibit tumorigenesis and is expressed at reduced levels in human breast carcinoma, suggesting that defects in autophagy proteins may contribute to the development or progression of tumors. PIST is a protein that interacts with Beclin-1 through its coiled-coil domain and can modulate Beclin-1 activity. LAMP1 and LAMP2 are homologous, highly glycosylated proteins associated with the lysosome and are thought to have overlapping functions. Mice lacking LAMP1 have very minor defects compared to those deficient in LAMP2 expression. However, the loss of both proteins results in embryonic lethality, suggesting that each protein possesses some unique and necessary functions. Rabbit polyclonal antibodies were raised against peptide sequences corresponding to each of the target proteins. Specificity: These polyclonal antibodies can be used for detection of APG7, Beclin-1, LAMP1, LAMP2 and PIST by immunoblot at 1-2ug/ml and their respective proteins via immunohistochemistry at 1-10ug/ml. Kit Components: APG7, CT (Rb x Hu): 1x25ug Beclin-1, CT (Rb x Hu): 1x25ug LAMP1 (Rb x Hu): 1x25ug LAMP2 (Rb x Hu): 1x25ug PIST, CT (Rb x Hu): 1x25ug Applications: Suitable for use in Western Blot and Immunohistochemistry. Other applications not tested. Recommended Dilution: Western Blot: 1-2ug/ml Immunohistochemistry: 1-10ug/ml Optimal dilutions to be determined by the researcher. Positive Control: Anti-Beclin-1: A431 Whole Cell Lysate Storage and Stability: May be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.