The Avidin/Biotin blocking system is designed to minimize the non-specific staining seen in some tissues due to endogenous avidin, biotin or biotin binding factors, when using avidin/biotin or streptavidin/biotin detection systems. Tissue sections should be incubated for 15 minutes with the avidin solution, washed well, and then incubated with the biotin solution for a further 15 minutes. Following further washing the primary antibody may then be added. We recommend that any blocking steps with serum or to block endogenous peroxidase are performed before avidin/biotin blocking.
Biological Properties: The Avidin/Biotin blocking system is designed to minimize the non-specific staining seen in some tissues due to endogenous avidin, biotin or biotin binding factors, when using avidin/biotin or streptavidin/biotin detection systems.Tiss
Contents: 30ml (1x15ml Avidin solution, 1x15ml Biotin solution)
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