B16 Cell Lysate, Murine

Transplantable Murine melanomas are well-established models for the study of experimental cancer therapies. Melanomas express different tumor-associated Ags, which are potential targets for novel designs of therapeutic cancer vaccines. Melanoma-associated Ags include tyrosinase and tyrosine-related protein (TRP)-1/2 or the MAGE and BAGE class of Ags. Many immunapeutic protocols have been tested using the Murine B16 melanoma cell line (and its sublines) that originated in the syngeneic C57BL/6 (H-2b) Murine strain. B16 melanoma cells express some of these melanoma-associated Ags, and their growth can be controlled in vivo by different classes of specific or nonspecific immune effector cells. The B16 cell line contains a large amount of PDGF b-receptor which makes it a suitable positive control for PDGF b-receptor.\n\nApplication: \nSuitable for use as a control in Western blot and the Electrophoretic Mobility Shift Assay (EMSA). Other applications not tested.\n\nRecommended Dilution:\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nAliquot to avoid repeated freezing and thawing and freeze at -70 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquots are stable for at least 12 months.

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