b2-Microglobulin (b2M) BioAssay(TM) ELISA Kit

Human Beta-2 Microglobulin (B2MG) is an 11.8kD protein identical to the light chain of the HLA-A, -B, and -C antigen. B2MG is expressed on nucleated cells, and is found at low levels in the serum and urine of normal individuals. B2MG concentrations are increased in inflammatory diseases, some viral diseases, renal dysfunction, and autoimmune diseases. A number of publications are available which explain the interpretation of B2MG serum levels in assessing the status of individuals with various clinical conditions. The enzyme immunoassay allows the quantitative determination of B2MG from serum. In this assay, the B2MG in the samples is bound to an available excess of monoclonal antibodies against B2MG, which are immobilized to the surface of the microtiter wells. After a washing step to remove all foreign substances, the quantification of bound B2MG is carried out by adding an enzyme (horseradish peroxidase or HRPO) labeled antibody, which also binds to the B2MG. The amount of bound enzyme is directly proportional to the B2MG content. The substrate is then converted to a chromogenic compound, which can be determined photometrically at 450 nm.\n\nDetection Range: 0-20 pg/ml\n\nSpecificity: 96%\n\nSensitivity: 0.5 pg/ml\n\nIntended Use: \nThe Beta-2 Microglobulin EIA Test is an enzyme immunoassay (EIA) for the measurement of Beta-2 Microglobulin in serum as an aid in the diagnosis of active rheumatoid arthritis and kidney disease.\n\nKit Components:\nMicrotiter Plate, 1x96wells\nSample Diluent, 1x100ml \nEnzyme conjugate reagent, 1x22ml \nB2MG reference standards, 1 set (liquid)\nTMB Substrate, 1x12ml \nStop Solution, 1x12ml\nWash Buffer Concentrate (50X), 1x15ml\n\nStorage and Stability:\nStore all components at 4 degrees C. Stable for 6 months. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

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SPECIFICATIONS

Catalog Number

167561

Size

96Tests

Applications

ELISA

References

1. Berggard I and Beam AG: 1968. Isolation and properties of a low molecular weight b2-globulin occurring in human biological fluids. J Biol Chem 243: 4095-4103. 2. Grey HM, Kubo RT, Colon SM, Poulik MD, Cresswell P, Springer T, Turner M and Strominger JL: 1973. The small subunit of HL-A antigens is b2-microglobulin. J Exp Med 138: 1608-1612. 3. Nakamuro K, Tanigaki N and Pressman D: 1973. Multiple common properties of human b2- microglobulin and the common portion fragment derived from HL-A antigen molecules. Proc Natl Acad Sci 70: 2863-2865. 4. Evrin PE and Wibell L: 1972. The serum levels and urinary excretion of b2-microglobulin in apparently healthy subjects. Scand J Clin Lab Invest 29: 69-74. 5. Crisp AJ, Coughlan RJ, Mackintosh D, Clark B and Panayi, GS: 1983. b2-microglobulin plasma levels reflect disease activity in rheumatoid arthritis. J Rheumatol 10: 954-956.