b2-Microglobulin (B2M) BioAssay(TM) ELISA Kit

Human Beta-2 Microglobulin (B2M) is a small (11kD) polypeptide that forms the invariant subunit of the class I HLA-antigens (Human Lymphocyte Antigen) on the cell membrane (1). Increased concentrations of B2M in serum have been found in patients with renal diseases, malignancies (especially of lymphatic origin) and infectious diseases, e.g., AIDS. B2M is the most powerful single prognostic variable in multiple myelome, it is also a good predictor for acute rejection in renal transplantation, furthermore the measurement of B2M is very useful for detecting and monitoring the changes in the glomerular filtration rate in diabetic nephropathy.\n\nSensitivity: \n~0.5ng/ml\n\nAssay Time: \n90 min.\n\nKit Components:\nM5891-01: Microtiter Plate 1x96wells\nM5891-02: Sample Diluent 1x50ml\nM5891-03: Standard 2.5ng/ml 1x1ml\nM5891-04: Standard 5ng/ml 1x1ml\nM5891-05: Standard 20ng/ml 1x1ml\nM5891-06: Standard 50ng/ml 1x1ml\nM5891-07: Standard 150ng/ml 1x1ml\nM5891-08 :B2M (HRP) 1x11ml\nM5891-09: Wash Buffer (20X) 1x50ml\nM5891-10: HRP Substrate A 1x11ml\nM5891-11: HRP Substrate B 1x11ml\nM5891-12: Stop Solution 1x10ml\n\nStorage and Stability:\nStore at components except standards at 4 degrees C. Store opened Standards M5891-03-M5891-07 as aliquots at -20 degrees C. Stable for 6 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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SPECIFICATIONS

Catalog Number

M5891

Size

1Kit

Applications

ELISA

Reactivities

Hum

References

1. Viedma, J.A., et al., Clin. Chem. 12: 2464 (1992). 2. Bernard, A.M., Clin. Chim. Acta 126: 1 (1982). 3. Tienhara, A., Clin. Chem. 36: 1961 (1990). 4. Hemmingsen, L., Clin. Lab Invest. 45: 367 (1985). 5. Bjerrum, O.W., Clin. Chim. Acta 155: 69 (1986).