B4GALT5, CT (B4GALT5, Beta-1,4-galactosyltransferase 5, Beta-1,4-GalT II, UDP-Gal:beta-GlcNAc beta-1,4-galactosyltransferase 5, UDP-galactose:beta-N-acetylglucosamine beta-1,4-galactosyltransferase 5) (AP)

This gene is one of seven beta-1,4-galactosyltransferase (beta4GalT) genes. They encode type II membrane-bound glycoproteins that appear to have exclusive specificity for the donor substrate UDP-galactose; all transfer galactose in a beta1,4 linkage to similar acceptor sugars: GlcNAc, Glc, and Xyl. Each beta4GalT has a distinct function in the biosynthesis of different glycoconjugates and saccharide structures. As type II membrane proteins, they have an N-terminal hydrophobic signal sequence that directs the protein to the Golgi apparatus and which then remains uncleaved to function as a transmembrane anchor. By sequence similarity, the beta4GalTs form four groups: beta4GalT1 and beta4GalT2, beta4GalT3 and beta4GalT4, beta4GalT5 and beta4GalT6, and beta4GalT7. The function of the enzyme encoded by this gene is not clear. This gene was previously designated as B4GALT4 but was renamed to B4GALT5. In the literature it is also referred to as beta4GalT2. [provided by RefSeq].\n\nApplications:\nSuitable for use in Western Blot, Flow Cytometry, ELISA\n\nRecommended Dilution:\nELISA: 1:1,000\nWestern Blot: 1:100-500\nFlow Cytometry: 1:10-50\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Do not freeze alkaline phosphatase conjugates which could result in a substantial loss of enzymatic activity. Light sensitive. Aliquots are stable for 6 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Prices direct from United States Biological

Quick response times

Exclusive Biosave savings/discounts

SPECIFICATIONS

Catalog Number

032365-AP

Size

200ul

Applications

ELISA, FC, WB

Hosts

Rabbit

Reactivities

Hum, Mouse

Form

Supplied as a liquid in PBS, pH 7.2. Contains ~0.09% sodium azide. Conjugated to Alkaline Phosphatase.

P Type

Pab

Purity

Purified by Protein A affinity chromatography.

Isotype

IgG

References

Kitayama, K., et al. J. Biol. Chem. 282(41):30085-30096(2007)\nSato, T., et al. J. Biol. Chem. 282(38):27702-27712(2007)\nJiang, J., et al. J. Biol. Chem. 281(14):9482-9489(2006)\nSato, T., et al. J. Biol. Chem. 279(38):39574-39583(2004)\nXu, S., et al. J. Exp. Clin. Cancer Res. 21(3):409-414(2002)\nDeloukas, P., et al. Nature 414(6866):865-871(2001)\nAmado, M., et al. Biochim. Biophys. Acta 1473(1):35-53(1999)\nLo, N.W., et al. Glycobiology 8(5):517-526(1998)\nSato, T., et al. Biochem. Biophys. Res. Commun. 244(3):637-641(1998)\nSato, T., et al. Proc. Natl. Acad. Sci. U.S.A. 95(2):472-477(1998)

Additional Info

Human, mouse

Alternative Names

B4GALT5, Beta-1,4-galactosyltransferase 5, Beta-1,4-GalT II, UDP-Gal:beta-GlcNAc beta-1,4-galactosyltransferase 5, UDP-galactose:beta-N-acetylglucosamine beta-1,4-galactosyltransferase 5