BACE2 (Beta-Site APP Cleaving Enzyme 2, Asp1, Aspartyl protease 1, Memapsin 1, Membrane-anchored aspartic protease of the pepsin family)

BACE-2 (b-site APP cleaving enzyme 2; also ASP-1 and Memapsin-1) is a 73-75kD member of the peptidase A1 family. It is expressed both inside and outside the CNS and is classified as a Golgi aspartyl protease. Its status as a b-secretase is unclear. If it has physiological activity, it may be associated with glia. Mature BACE-2 is a 456 amino acid (aa) type I transmembrane glycoprotein. It contains a 411 aa extracellular domain (aa 63-473) that has two catalytic residues (Asp 93 and Asp 289). Two potential splice forms exist; one shows a deletion of aa's 329-378, while a second shows an 18 aa substitution for aa's 380-518. Over aa 63-466, human BACE-2 shares 92% aa sequence identity with mouse BACE-2. \n \nApplications:\nWestern Blot: This antibody can be used at 0.1-0.2ug/ml with the appropriate secondary reagents to detect human and mouse BACE-2. The detection limit for BACE-2 is approximately 5ng/lane and 25 ng/lane under non-reducing and reducing conditions, respectively.\nImmunoprecipitation: This antibody has been used to immunoprecipitate rhBACE-2 from conditioned media of transfected NS0 cells. \nDirect ELISA: This antibody can be used at 0.5-1.0ug/ml with the appropriate secondary reagents to detect human and mouse BACE-2. The detection limit for BACE-2 is approximately 0.5 ng/well. \nOptimal dilutions should be deterhined by each laboratory for each application.\n\nStorage and Stability:\nLyophilized powder may be stored at 4 degrees C for short-term only. Reconstitute to nominal volume by adding sterile PBS with 40-50% glycerol and store at -20 degrees C. Reconstituted product is stable for 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

B0002-91J

Size

100ug

Applications

ELISA, IP, WB

Hosts

Sheep

Reactivities

Hum

Form

Supplied as a lyophilized powder from a 0.2um filtered solution in PBS with 5% trehalose. Reconstitute with sterile PBS, 40-50% glycerol. If 0.5ml is used, the concentration will be 0.2mg/ml.

P Type

Pab

Purity

Purified by human BACE-2 affinity chromatography.

Isotype

IgG

Additional Info

Selected for its ability to recognize human BACE-2 in direct ELISAs and Western Blots. In these formats, this antibody shows less than 1% crossreactivity with rhBACE-1, rhCathepsin D and rhCathepsin E.