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BAF57 (SMARCE1, FLJ35648, SWI/SNF-related matrix-associated actin-dependent regulator of chromatin subfamily E member 1, BRG1-associated factor 57)

Cat no: 045565

BAF57 (SMARCE1, FLJ35648, SWI/SNF-related matrix-associated actin-dependent regulator of chromatin subfamily E member 1, BRG1-associated factor 57)

BAF57 is part of the large ATP-dependent chromatin remodeling complex SWI/SNF, which is required for activation of proteins normally repressed by chromatin. It, either alone or when in the SWI/SNF complex, can bind to 4-way junction DNA, which is thought to mimic the topology of DNA as it enters or exits the nucleosome. BAF57 contains a DNA-binding HMG domain, but disruption of this domain does not abolish the DNA-binding or nucleosome-displacement activities of the SWI/SNF complex. Unlike most of the SWI/SNF complex proteins, BAF57 has no yeast counterpart (1).\n\nApplications: \nSuitable for use in Western Blot, Immunoprecipitation, Immunohistochemistry, Immunocytochemistry. Other applications not tested.\n\nRecommended Dilution:\nWestern Blot: 1:1000-10,000\nImmunohistochemistry: 1:100-250\nImmunocytochemistry: 1:100-250\nImmunoprecipitation: 1:10-100\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. \n\nManufactured incorporating RabMAb(R) technology under Epitomics US patents, No 5,675,063 and 7,429,487, owned by Abcam.

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SPECIFICATIONS

Catalog Number

045565

Size

100ul

Applications

ICC, IHC, IP, WB

Hosts

Rabbit

Reactivities

Hum, Mouse, Rat

Form

Supplied as a liquid in PBS, sodium azide, BSA, glycerol.

P Type

Mab

Purity

Supernatant

References

1. Entrez Gene: gene-centered information at NCBI. Nucleic Acids Res. 2005 Jan 1;33:D54-8.

Additional Info

Recognizes human BAF57. Species sequence homology: Mouse, rat.

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SUPPLIER INFO

Applications

ELISA

Reactivities

Hum

More info

Applications

IF

Hosts

Mouse

More info

Applications

ELISA, WB

Hosts

Mouse

Reactivities

Hum

More info

Applications

ELISA, FC, WB

Hosts

Mouse

Reactivities

Hum

More info

Applications

ELISA, FC, IHC, WB

Hosts

Mouse

More info

Applications

IHC, WB

Hosts

Rabbit

Reactivities

Hum

More info

Applications

ELISA, WB

Hosts

Rabbit

Reactivities

Hum

More info

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