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Bcr (Breakpoint Cluster Region Protein, Renal Carcinoma Antigen NY-REN-26, BCR1, D22S11)

Cat no: C0013-01C

Bcr (Breakpoint Cluster Region Protein, Renal Carcinoma Antigen NY-REN-26, BCR1, D22S11)

The Bcr gene was orginally identified by its presence in the chimeric Bcr-Abl oncogene (1). The N-terminal part of Bcr contains an oligomerization domain, a serine/threonine kinase domain and a region that binds SH2 domain. The middle of the protein has a PH domain and a region of sequence similarity to the guanine nucleotide exchange factors for the Rho family of GTP binding proteins. The C-terminal part may be involved in a GTPase-activating function for the small GTP-binding protein Rac (2,3). The function of Wildtype Bcr in cells remains unclear. PDGF receptor may use Bcr as a downstrean signal mediator (4). The Bcr-Abl fusion results in production of a constitutively active tyrosine kinase, which causes chronic myelogenous leukemia (CML) (5). Tyrosine 177 of Bcr is phosphorylated in Bcr-Abl fusion protein, which plays an important role in transforming the activity of Bcr-Abl \n(6). Phosphorylated tyrosine 177 of Bcr provides a docking site for Gab2 and GRB2 (7,8).\n\nApplications: \nSuitable for use in ELISA, Flow Cytometry, Western Blot and Immunocytochemistry. Other applications not tested.\n\nRecommended Dilution:\nWestern Blot: 1:1000\nImmunocytochemistry: 1:100\nFlow Cytometry: 1:25\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. For long-term storage, store at -20 degrees C. Aliquots are stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

C0013-01C

Size

100ul

Applications

ELISA, FC, ICC, WB

Hosts

Rabbit

Reactivities

Hum, Mouse, Rat

Form

Supplied as a liquid in 10mM sodium HEPES, pH 7.5, 150mM sodium chloride, BSA, 50% glycerol.

P Type

Pab

Purity

Purified by protein A and immunoaffinity chromatography.

Isotype

IgG

References

(1) Groffen, J. et al. (1984) Cell 36, 93

Additional Info

Detects endogenous levels of total Bcr and the 210kD Bcr-Abl fusion protein. Species Crossreactivity: Human, mouse and rat.

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