Beclin 1 mAb

BECN1 (beclin 1) is a coiled-coil protein that has been implicated as an\r inhibitor of tumorigenesis. BECN1, which associates with Bcl-2, plays a\r significant role in autophagy. Autophagy is the degradation of cellular proteins\r in the lysosomes, and when this pathway is suppressed, cell growth is\r deregulated. Autophagy is controlled by the same signal transduction pathway\r that induces the phosphorylation of the ribosomal protein S6, and both\r are mediated via amino acids. BECN1 expression in various carcinoma cell\r lines, such as MCF7, is low, whereas it is ubiquitously expressed in normal\r breast tissue. In transfected MCF7 cells, BECN1 complements autophagocytosis\r and, subsequently, inhibits cellular proliferation. Additionally, BECN1\r shares structural similarity to the yeast autophagy gene product, APG6, and\r was one of the first mammalian proteins discovered to mediate autophagy.\r

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SPECIFICATIONS

Catalog Number

MB0030

Size

100ug/100ul

Applications

WB

Hosts

Mouse

Reactivities

Hum, Mouse

Form

liquid

Purity

The antibody was affinity-purified from mouse ascites by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Antigen

Recombinant full length Human Beclin 1.

Species

This antibody detects endogenous levels of Beclin 1 and does not cross-react with related proteins

Dilutions

WB: 1:1000

Swiss Prot

Q14457

Storage Temp

Store at 4 degrees C short term. Aliquot and store at -20 degrees C long term. Avoid freeze-thaw cycles.

Concentration

1ug/ul

Storage Buffer

1 mg/ml in Phosphate buffered saline (PBS) with 0.05% sodium azide, approx. pH 7.2.

Additional Info

For research use only, not for use in diagnostic procedure.

Molecular Weight

Predicted band size:52KDa \nObserved band size:55KDa

Alternative Names

APG6; \r\nATG 6; \r\nATG6; \r\nATG6 autophagy related 6 homolog; \r\nBcl-2-interacting protein beclin; \r\nBeclin 1 (coiled coil moesin like BCL2 interacting protein); \r\nBeclin 1 autophagy related; \r\nBeclin-1; \r\nBeclin1; \r\nBECN 1; \r\nBECN1; \r\nBECN1; \r\nCoiled coil myosin like BCL2 interacting protein; \r\nCoiled-coil myosin-like BCL2-interacting protein; \r\nGT197; \r\nProtein GT197 antibod