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BID, CT (BH3-interacting Domain Death Agonist, BH3-interacting Domain Death Agonist p11, p11 BID, BH3 interacting domain death agonist p13, p13 BID, BH3 interacting domain death agonist p15, p15 BID, BID Isoform L(2), Apoptotic Death Agonist, Desmocollin

Cat no: B1095-08

BID, CT (BH3-interacting Domain Death Agonist, BH3-interacting Domain Death Agonist p11, p11 BID, BH3 interacting domain death agonist p13, p13 BID, BH3 interacting domain death agonist p15, p15 BID, BID Isoform L(2), Apoptotic Death Agonist, Desmocollin

BID contains a BH3 domain, which is required for its interaction with the Bcl-2 family proteins and for its pro-death activity. BID is susceptible to proteolytic cleavage by caspases, calpains, Granzyme B and cathepsins. Protease-cleaved BID translocates to mitochondria where it results in cytochrome c release, leading to caspase activation and cell death. This constitutes the activation of the mitochondria death pathway (the intrinsic pathway) by the death stimuli. \n\nApplications:\nSuitable for use in ELISA and Western Blot. Other applications not tested.\n\nRecommended Dilution:\nELISA: 1:8000\nWestern Blot: 0.1-0.3ug/ml\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile 40-50% glycerol, aliquot and store at -20 degrees C. Aliquots are stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

B1095-08

Size

100ug

Applications

ELISA, WB

Hosts

Goat

Reactivities

Hum

Form

Supplied as a liquid in Tris-saline, pH 7.3, 0.02% sodium azide, 0.5% BSA.

P Type

Pab

Purity

Purified by immunoaffinity chromatography.

Isotype

IgG

References

1. Yin, X.M. (2006) Bid, a BH3-only multi-functional molecule, is at the cross road of life and death. Gene. 369: 7-19.

Additional Info

Recognizes human BID (C-TERMINAL).

Alternative Names

BID Isoform ES(1b)

Read more on Supplier website

SUPPLIER INFO

United States Biological

Applications

ELISA

Reactivities

Hum

More info

Applications

IF

Hosts

Mouse

More info

Applications

ELISA, WB

Hosts

Mouse

Reactivities

Hum

More info

Applications

ELISA, FC, WB

Hosts

Mouse

Reactivities

Hum

More info

Applications

ELISA, FC, IHC, WB

Hosts

Mouse

More info

Applications

IHC, WB

Hosts

Rabbit

Reactivities

Hum

More info

Applications

ELISA, WB

Hosts

Rabbit

Reactivities

Hum

More info
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