BLC, Recombinant, Mouse (B-lymphocyte Chemoattractant, Mouse Equivalent of Human B Cell-Attracting Chemokine-1, BCA-1, CXCL13)

BCA-1/BLC, a CXC chemokine, is expressed in the liver, spleen, lymph nodes, appendix and stomach. It exerts its activities through its only receptor CXCR5. BCA-1/BLC is a potent chemoattractant for B lymphocytes and induces weak chemotactic response in T cells and macrophages. It manifests no activity on neutrophils and monocytes.\n\nSource: \nRecombinant corresponding to 86aa from mouse BLC expressed in E. coli.\n\nMolecular Weight: \n~9.8kD\n\nEndotoxin: (same/less than)0.1ng/ug\n\nBiological Activity: \nThe biological activity was determined by measuring dose-dependent chemotaxis with murine B lymphocytes. A concentration range of 10-100ng/ml produced significant chemotaxis. BLC, a CXC chemokine, regulates B cell migration to and within lymphoid follicles in most secondary lymphoid organs. The receptor for BLC is CXCR5.\n\nStorage and Stability:\nStore at -20 degrees C. Reconstitute to nominal volume by adding sterile ddH2O, 0.1-1% BSA. Aliquot and store at -20 degrees C. Reconstituted product is stable for 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

B0395-07A

Size

20ug

Form

Supplied as a sterile filtered lyophilized powder, carrier free. Reconstitute in sterile, ddH2O, 0.1-1% BSA to 0.5-1mg/mL. Further dilutions should be made in aqueous buffers.

Purity

~98% (SDS-PAGE, HPLC). Purified by sequential chromatography.