BLVRB (Biliverdin Reductase B, Flavin Reductase (NADPH), FR, BVR-B, Biliverdin-IX beta-reductase, Green Heme-binding Protein, GHBP, NADPH-dependent Diaphorase, NADPH-flavin Reductase, FLR)

Clearance of heme in mammals is a two step process starting with conversion of heme to biliverdin by heme oxygenase, followed by reduction of biliverdin to bilirubin by bilivredin reductase. Biliverdin IX b reductase (BLVRB) converts the B isomer of biliverdin IX to bilirubin IX b, which constitutes 87% of total bilirubin in fetal bile. Therefore BLVRB is especially important for fetal heme metabolism and clearance. BLVRB is a cytoplasmic enzyme expressed at high levels in erythrocytes and liver, but is present in other tissues. The enzyme is identical to flavin reductase, which is an oxidoreductase that catalyses the NADPH-dependent reduction for a variety of flavins, such as riboflavin, FAD or FMN and methemoglobin. BLVRB is structurally distinct from BLVRA. In contrast to BLVRA, which prefers the biliverdin a isomer but could also use the B isomer as substrate, BLVRB is specific for the B isomer.\n\nApplications:\nSuitable for use in Western Blot. Other applications not tested.\n\nRecommended Dilution:\nWestern Blot: 1ug/ml\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nLyophilized powder may be stored at -20 degrees C. Stable for 12 months at -20 degrees C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Reconstituted product is stable for 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

B1150-03M

Size

100ug

Applications

WB

Hosts

Sheep

Reactivities

Hum, Mouse, Rat

Form

Supplied as a lyophilized powder in PBS, trehalose. Reconstitute with sterile PBS to 0.2mg/ml.

P Type

Pab

Purity

Purified by immunoaffinity chromatography.

Isotype

IgG

References

1. Pereira, P.J. et al. (2001) Nat. Struct. Biol. 8:215. 2. Chikuba, K. et al. (1994) Biochem. Biophys. Res. Commun. 198:1170. 3. Shalloe, F. et al. (1996) Biochem. J. 316:385. 4. Cunningham, O. et al. (2000) Biochem. J. 345:393. 5. Yamaguchi, T. et al. (1994) J. Biol. Chem. 269:24343. 6. Cunningham, O. et al. (2000) J. Biol. Chem. 275:19009.

Additional Info

Recognizes human BLVRB. Species Crossreactivity: mouse and rat.