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BMP8B (Bone Morphogenetic Protein 8B, BMP-8B)

Cat no: B2553-35X

BMP8B (Bone Morphogenetic Protein 8B, BMP-8B)

BMP-8b is a 16kD predicted mature and 45kD precursor member of the Gbb-60A class of the BMP family of molecules. In mouse, precursors to sperm and oocytes arise from the proximal epiblast around day E7.5 (days 18-20 in human). This lineage commitment is a response to BMP-4 signaling, which is promoted by the repressive action of BMP-8b on the anterior visceral endoderm, a source of BMP-4 antagonism. Mouse BMP-8b preproprecursor is 399aa in length. It contains a 19aa signal sequence, a 241aa prosegment, and a 139aa mature region. An interchain disulfide bond occurs at 363aa, generating a homodimer. Over aa261-399, mouse BMP-8b shares 74% aa identity with mouse BMP-8a, and 69% and 93% aa identity with human and rat BMP-8b.\n\nApplications:\nSuitable for use in Western Blot. Other applications not tested.\n\nRecommended Dilution:\nWestern Blot: 1ug/ml\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nLyophilized powder may be stored at -20 degrees C. Stable for 12 months at -20 degrees C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Reconstituted product is stable for 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

B2553-35X

Size

100ug

Applications

WB

Hosts

Sheep

Reactivities

Mouse

Form

Supplied as a lyophilized powder in PBS, trehalose. Reconstitute with sterile PBS to 0.2mg/ml.

P Type

Pab

Purity

Purified by immunoaffinity chromatography.

Isotype

IgG

Additional Info

Recognizes mouse BMP-8b. Species sequence homology: Recombinant human BMP-3b, rhBMP-10, and recombinant mouse BMP-10 human (10%).

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Applications

ELISA

Reactivities

Hum

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Applications

IF

Hosts

Mouse

More info

Applications

ELISA, WB

Hosts

Mouse

Reactivities

Hum

More info

Applications

ELISA, FC, WB

Hosts

Mouse

Reactivities

Hum

More info

Applications

ELISA, FC, IHC, WB

Hosts

Mouse

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Applications

IHC, WB

Hosts

Rabbit

Reactivities

Hum

More info

Applications

ELISA, WB

Hosts

Rabbit

Reactivities

Hum

More info
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