BP-1 (Ly-51, 6C3) (Biotin)

BP-1, also known as Ly-51/6C3 antigen, is a type II homodimeric transmembrane glycoprotein formed by two disulfide-linked chains of 140 kD.1, 2 Originally identified on the surface of pre-B cell lymphomas1, 3 the cDNA encoding BP-1/Ly-51/6C3 has been cloned and found to represent aminopeptidase A.4, 5 It is expressed at high levels on bone marrow stromal cell lines which support in vitro B lymphopoiesis and on a wide variety of mouse tissues known to possess aminopeptidase activity.6-8 Subsets of normal bone marrow pre-B and B cells express low levels of BP-1/Ly-51/6C3, which is rapidly upregulated on pre-B cells in the presence of IL- 7.9, 10 The BP-1/6C3 marker is commonly used to identify B cells in different stages of development.11\n\nApplications:\nSuitable for use in Flow Cytometry. Other applications not tested.\n\nRecommended Dilution:\nFlow Cytometry: (same/less than) 1ug/10e6 cells\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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SPECIFICATIONS

Catalog Number

B2628-50B

Size

500ug

Applications

FC

Hosts

Rat

Reactivities

Mouse

Conjugates

Biotin

Form

Supplied as a liquid in PBS, 0.1% sodium azide. Conjugated to Biotin.

P Type

Mab

Purity

Purified

Isotype

IgG1,k

References

1. Cooper, M. D., D. Mulvaney, A. Coutinho, and P. A. Cazenave. 1986. Nature 321:616.\n2. Wu, Q., G. F. Tidmarsh, P. A. Welch, J. H. Pierce, I. L. Weissman, and M. D. Cooper. 1989. J. Immunol. 143:3303.\n3. Pillemer, E., C. Whitelock, and I. L. Weissman. 1984. Proc. Natl. Acad. Sci. USA. 81:4434.\n4. Wu, Q., J. M. Lahti, G. M. Air, P. D. Burrows, and M. D. Cooper. 1990. Proc. Natl. Acad. Sci. USA. 87:993.\n5. Wu, Q., L. Li, M. D. Cooper, M. Pierres, and J. P. Gorvel. 1991. Proc. Natl. Acad. Sci. U. S. A. 88:676.\n6. Whitlock, C. A., G. F. Tidmarsh, C. Muller-Sieburg, and I. L. Weissman. 1987. Cell 48:1009.\n7. Adkins, B., G. F. Tidmarsh, and I. L. Weissman. 1988. Immunogenetics 27:180.\n8. Li, L., Q. Wu, J. Wang, R. P. Bucy, and M. D. Cooper. 1993. Tissue Antigens. 42:488.\n9. Sherwood, P. J. and I. L. Weissman. 1990. Int. Immunol. 2:399.\n10. Welch, P. A., P. D. Burrows, A. Namen, S. Gillis, and M. D. Cooper. 1996. Int. Immunol. 2:697.\n11. Hardy, R. R., C. E. Carmack, S. A. Shinton, J. D. Kemp, and K. Hayakawa. 1991. J. Exp. Med. 173:1213.\n12. Shu, F. and J. F. Kearney. 1996. Personal communication.

Additional Info

Recognizes BP-1/Ly-51/6C3 antigen, (aminopeptidase A, E.C.3.4.11.7, glutamylaminopeptidase).