Bromodeoxyuridine (BrdU) (HRP)

Bromodeoxyuridine (BrdU) is a thymidine analog and is specifically incorporated into DNA during DNA synthesis. Detection of bromodeoxyuridine (BrdU) incorporated into DNA is a powerful tool to study the cytokinetics of normal and neoplastic cells. In vitro or in vivo labeling of tumor cells with the thymidine analogue BrdU and the subsequent detection of incorporated BrdU with specific anti-BrdU monoclonal antibodies is an accurate and comprehensive method to quantitate the degree of DNA-synthesis. BrdU is incorporated into the newly synthezised DNA of the S-phase cells and can thus provide an estimate for the fraction of cells in S-phase. Also dynamic proliferative information (such as the S-phase transit rate and the potential doubling time) can be obtained, by means of bivariate BrdU/DNA flow cytometric analysis.\n\nApplications:\nSuitable for use in ELISA and Immunohistochemistry (paraffin). Other applications not tested.\n\nRecommended Dilution:\nELISA: 1:250-1:1000\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and store at -20 degrees C. Aliquots are stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. Labeled with horseradish peroxidase (HRP). Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. HRP conjugates are sensitive to light.

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SPECIFICATIONS

Catalog Number

B2850-01R

Size

500ug

Applications

ELISA, IHC

Hosts

Rat

Form

Supplied as a liquid in PBS, pH7.4, 0.01% thiomersal. Conjugated to HRP.

P Type

Mab

Purity

Purified by Protein G affinity chromatography.

Isotype

IgG2a

Additional Info

Recognizes BrdU incorporated into single stranded DNA, attached to a protein carrier and free BrdU. It does not cross react with thymidine but does react weakly with chlorodeoxyuridine.