BTLA (B- and T-lymphocyte Associated Protein, B- and T-lymphocyte Attenuator, BTLA1, CD272, CD272 Antigen, FLJ16065, MGC129743) (Biotin)

Human CD272 (BTLA, B and T Lymphocyte Attenuator) is a member of the Immunoglobulin superfamily and has sequence homology to PD-1 and CTLA4. It is expressed on T and B lymphocytes and other hemopoetic lineages. Engagement of this molecule by its ligand, CD270 (HVEM, a TNF superfamily member) can down regulate activated T and B cell responses. CD272 levels on antigen specific CD8+ T cells have been reported to decrease in viral specific, but not melanoma specific activated lines (1). Polymorphism of the CD272 molecule has been linked to rheumatoid arthritis (3). \n\nApplications:\nSuitable for use in ELISA and Flow Cytometry. Other applications not tested.\n\nRecommended Dilution:\nELISA: Capture\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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SPECIFICATIONS

Catalog Number

B5001-61D

Size

100ug

Applications

ELISA, FC

Hosts

Mouse

Reactivities

Hum

Conjugates

Biotin

Form

Supplied as a liquid in 50mM sodium phosphate, pH 7.5, 100mM potassium chloride, 150mM sodium chloride, 0.2% BSA, 0.04% sodium azide, 5% glycerol. Labeled with Biotin.

P Type

Mab

Purity

Purified by Protein A affinity chromatography.

Isotype

IgG1,k

References

1. Derre

Additional Info

Recognizes human CD270 (BTLA). Bnds to CD272 on the cell surface of human PBMC in Flow cytometry and to recombinant CD272-muIg in EIA. Blocks binding of Biotinylated CD270(HVEM)-muIg to CD272-muIg in EIA.

Alternative Names

B- and T-lymphocyte Associated