Cadherin E (Cadherin 1, E-Cadherin, L-CAM, Epithelial Cell Adhesion Molecule, Uvomorulin, CDH1)

E-cadherin (Uvomorulin, L-CAM, Epithelial Cell Adhesion Molecule, cell CAM 120/180) is a 123kD molecule of the cadherin family. One of the epithelial cell adhesion molecules, E-Cadherin, plays an important role in the formation of cell-cell contacts in epithelia irrespective their origin form ecto-, meso-or endodermal tissue. This early adhesion event between epithelial cells is a prerequisite for the assembly of all elements of the junctional complex. Furthermore, E-Cadherin plays a crucial role in the maintenance of the epithelial junctional complex and is as such an important molecule in maintaining epithelial integrity. Cadnerins constitute a familly of transmembrane glycoproteins involved in Ca2+ dependent cell-cell interactions. The members of this family are diferentially expressed in various tissues. One of the best characterized cadherins in E-cadherin, a 120 kD transmembrane glycoprotein consisting of an 80 kD extracellular and a 40kD transmembrane and cytoplasmic part. Cadherin E is a functional antibody which inhibits cell-cell adhesion. Antibodies against E-cadherin are suitable to characterize epithelial cells. E-Cadherin is involved in adhesion of epithelial cells (mostly homotypic and homophilic) and is expressed on immature erythroid cells. In addition, it blocks the interaction of E-cadherin molecules and therefore is suitable to study E-cadherin-mediated signal transduction. Cadherins are a family of CA2+ dependent intercellular adhesion molecules that play a central role in controlling morphogenetic movements during development. Their function is regulated by association with the actin cytoskeleton by a complex of cytoplasmic proteins called the catenin (alpha, beta and gamma). Members of the cadherin family include Cadherin 6 (K-Cadherin), P-cadherin, E-cadherin (uvomorulin), N-cadherin (neural cadherin), R-Cadherin, cadherin 5, L-CAM and EP-Cadherin. Applications: Suitable for use in Immunofluorescence, Immunohistochemistry (frozen sections), Western Blot and Immunoprecipitation. Other applications not tested. Recommended Dilution: Immunofluorescence: 1:1600 Western Blot: 1:3000 Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4 degrees C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot Store at -20 degrees C. Aliquots are stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.